5-Methylcytidine Antibody (33D3)

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, MeDIP
Clone
33D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

5-Methylcytidine Antibody (33D3) Summary

Immunogen
Made to Chemical 5-Methylcytidine.
Specificity
Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue. Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine. NB100-65014 has been reported for use in Methylated DNA immunoprecipitation (MeDIP).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Methylated DNA Immunoprecipitation 1:10-1:1000
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Flow Cytometry: Membrane permeabilization may be required prior to staining. Use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. Cell pretreatment before staining is described in Giraldo et al (2007)

Reactivity Notes

Cross reacts with Human, Rat, Mouse and Bovine May also react with a broad range of species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.01% Thimerosal
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for 5-Methylcytidine Antibody (33D3)

  • 5 MeCyd
  • 5 MethylCytidine
  • 5-MethylCytidine

Background

Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that is formed when cytosine is attached to a ribose ring (also known as a ribofuranose) via a beta-N1-glycosidic bond. Recognizes the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumors, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5-Methylcytidine Antibody (NB100-65014) (0)

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Secondary Antibodies

 

Isotype Controls

Additional 5-Methylcytidine Products

5-Methylcytidine NB100-65014

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