5-HT4 Antibody

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Western Blot: 5-HT4 Antibody [NBP1-01016] - Analysis of SR-4 (N180) antibody in extracts from NIH-3T3 cells.

Product Details

Summary
Product Discontinued
View other related 5-HT4 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-01016
    • Availability
      Product Discontinued

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5-HT4 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 146-198 of Human SR-4.
Clonality
Polyclonal
Host
Rabbit
Gene
HTR4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
Western blot (WB) analysis of SR-4 (N180) pAb in extracts from NIH-3T3 cells.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-HT4 Antibody

  • 5HT4
  • 5-HT4
  • 5-HT-4
  • 5-HT4R
  • 5-hydroxytryptamine (serotonin) receptor 4,5-hydroxytryptamine receptor 4,5-HT45-hydroxytryptamine4 receptor
  • cardiac 5-HT4 receptor
  • HTR4
  • Serotonin receptor 4

Background

Serotonin (also designated 5-hydroxytryptamine or 5-HT) is a molecule that functions as a neurotransmitter, a hormone and a mitogen, and it is predominantly expressed in the gut, platelets and central nervous system (CNS). In the CNS, serotonin modulates several processes, including anxiety, sleep, appetite, behavior and drug abuse. In platelets and gut, serotonin plays a major role in cardiovascular function and motility of the gastrointestinal tract, respectively. Serotonin mediates its effects through several of G protein coupled receptors, designated 5-HT receptors or alternatively SR receptors. SR-3 is a ligand-gated ion channel, whereas all other known serotonin receptor subtypes are G protein-coupled receptors. The gene which encodes SR-3 maps to human chromosome 11q23.1-q23.2. SR-4 mediates widespread effects in central and peripheral nervous systems. The gene which encodes SR-4 maps to human chromosome 5q31-q33. SR-7 belongs to the superfamily of G protein-coupled receptors. The gene which encodes SR-7 maps to human chromosome 10q21-q24.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HTR4
Entrez