4EBP1 Antibody

Images

 
Western Blot: 4EBP1 Antibody [NB100-92009] - Analysis of extracts from Jurkat cells treated with Insulin 0.01U/ml 15'.
Immunohistochemistry-Paraffin: 4EBP1 Antibody [NB100-92009] - Paraffin-embedded human breast carcinoma tissue at 1:100.
Immunohistochemistry-Paraffin: 4EBP1 Antibody [NB100-92009] - Analyzes of paraffin-embedded human colon carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related 4EBP1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92009
    • Availability
      Product Discontinued

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4EBP1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 26-76 of Human 4E-BP1.
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4EBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
4EBP1 Lysate (NBL1-10206)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 4EBP1 Antibody

  • 4EBP1
  • 4E-BP1
  • eIF4E-binding protein 1
  • EIF4EBP1
  • eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
  • eukaryotic translation initiation factor 4E-binding protein 1
  • PHAS-I
  • PHAS-IMGC4316
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein eIF4EBP1 Antibody, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to eIF4EBP1, designated 4E-BP2, also associates with eIF-4E.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Contact Information

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Bioinformatics

Gene Symbol EIF4EBP1
Entrez
Uniprot