4EBP1 Antibody


Western Blot: 4EBP1 Antibody [NB100-92009] - Analysis of extracts from Jurkat cells treated with Insulin 0.01U/ml 15'.
Immunohistochemistry-Paraffin: 4EBP1 Antibody [NB100-92009] - Paraffin-embedded human breast carcinoma tissue at 1:100.
Immunohistochemistry-Paraffin: 4EBP1 Antibody [NB100-92009] - Analyzes of paraffin-embedded human colon carcinoma tissue.

Product Details

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4EBP1 Antibody Summary

Synthetic peptide, corresponding to amino acids 26-76 of Human 4E-BP1.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
4EBP1 Lysate (NBL1-10206)
4EBP1 Lysate (NBP2-64630)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for 4EBP1 Antibody

  • 4EBP1
  • 4E-BP1
  • eIF4E-binding protein 1
  • EIF4EBP1
  • eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
  • eukaryotic translation initiation factor 4E-binding protein 1
  • PHAS-I
  • PHAS-IMGC4316
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1


The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein eIF4EBP1 Antibody, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to eIF4EBP1, designated 4E-BP2, also associates with eIF-4E.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol EIF4EBP1