4EBP1 Antibody (4F3-H2)

Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (4F3-H2) [H00001978-M01] - Analysis of monoclonal antibody to EIF4EBP1 on HeLa cell . Antibody concentration 10 ug/ml.

Proximity Ligation Assay: 4EBP1 Antibody (4F3-H2) [H00001978-M01] - Analysis of protein-protein interactions between BUB1 and EIF4EBP1. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-EIF4EBP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: 4EBP1 Antibody (4F3-H2) [H00001978-M01] - Detection limit for recombinant GST tagged EIF4EBP1 is 0.1 ng/ml as a capture antibody.

Product Discontinued

4EBP1 Antibody (4F3-H2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EIF4EBP1 (AAH04459, 1 a.a. ~ 118 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI
• Specificity: EIF4EBP1 - eukaryotic translation initiation factor 4E binding protein 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF4EBP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 4EBP1 Antibody (4F3-H2)

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4EBP1
• Entrez:
  • 1978(Human)
• OMIM:
  • 602223(Human)
• Uniprot:
  • AAH04459(Human)