26S proteasome subunit 9 Antibody

Product Discontinued

26S proteasome subunit 9 Antibody Summary

• Immunogen: Synthetic peptide corresponding to the C-terminal of human p27.
• Localization: Nuclear
• Specificity: p27
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMD9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1/200
• Control:

  Jurkat Whole Cell Lysate
  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate
  26S proteasome subunit 9 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human, Rat and Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 1 ml PBS containing 0.2% gelatin
• Preservative: 0.1% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: IgG purified

Alternate Names for 26S proteasome subunit 9 Antibody

• 26S proteasome non-ATPase regulatory subunit 9
• p27
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Rpn4

Background

The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMD9
• Entrez:
  • 5715(Human)
  • 161475(Rat)