15-PGDH/HPGD Antibody [FITC]

Product Discontinued

15-PGDH/HPGD Antibody [FITC] Summary

• Immunogen: Purified type I human placental 15-PGDH protein [UniProt# P15428]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HPGD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: This 15-PGDH antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. In Western blot bands may be seen at 29 kDa and 58 kDa (homodimer) depending on the lysate used. In ICC/IF cytoplasmic staining was observed in HeLa cells.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27140190)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for 15-PGDH/HPGD Antibody [FITC]

• 15-hydroxyprostaglandin dehydrogenase [NAD+]
• 15PGDH
• 15-PGDH
• EC 1.1.1
• EC 1.1.1.141
• HPGD
• hydroxyprostaglandin dehydrogenase 15-(NAD)
• NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
• PGDH1
• PGDH1PGDH
• Prostaglandin dehydrogenase 1
• SDR36C1
• short chain dehydrogenase/reductase family 36C, member 1

Background

15-PGDH (15-hydroxyprostaglandin dehydrogenase, NAD+ or HPGD) belongs to short-chain dehydrogenases/reductases (SDR) family and is a key enzyme responsible for biological inactivation of prostaglandins as well as related eicosanoids. 15-PGDH has a potential to catalyze NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4, and it brings about prostaglandin inactivation for regulating biological processes which are controlled by prostaglandin levels. It is down-regulated by cortisol, dexamethasone and betamethasone, and TGFB1. 15-PGDH is considered as a tumor suppressor and its decreased expression is observed in cancers subjects with colorectal, breast, prostate, lung, thyroid, gastric and other cancers. Furthermore, defects in 15-PGDH gene have been linked to diseases including hypertrophic osteoarthropathy, primary, autosomal recessive, type 1 (PHOAR1), cranioosteoarthropathy (COA), isolated congenital nail clubbing (ICNC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HPGD
• Entrez:
  • 3248(Human)
  • 15446(Mouse)
  • 79242(Rat)