15-Lipoxygenase 1 Antibody (OTI3G8)

Images

 
Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - COS7 cells
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Western Blot: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Western Blot: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, Ca, Mk, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

15-Lipoxygenase 1 Antibody (OTI3G8) Summary

Immunogen
Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ALOX15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
74.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-01740 in the following applications:

Publications
Read Publications using
NBP2-01740 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for 15-Lipoxygenase 1 Antibody (OTI3G8)

  • 15-lipooxygenase-1
  • 15-LOX-1
  • 15LOX-1
  • arachidonate 15-lipoxygenase
  • Arachidonate omega-6 lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.33
  • LOG15,15-LOX

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 15-Lipoxygenase 1 Antibody (NBP2-01740)(2)

Review for 15-Lipoxygenase 1 Antibody (NBP2-01740) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-01740:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot 15-Lipoxygenase 1 NBP2-01740
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reviewed by:
Anonymous
WB Human 09/10/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman retinal endothelial cells and U937
SpeciesHuman
LotA001

Blocking

Blocking Details5% non fat Milk for 1 hour at room temp.

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-mouse
Secondary Manufacturer Cat#Cell signaling, 7076s
Secondary Concentration1:5000

Details

Detection NotesX-ray, using supersignal west pico chemiluminescent kit

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 15-Lipoxygenase 1 Antibody (NBP2-01740) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for 15-Lipoxygenase 1 Antibody (NBP2-01740)

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Research Areas for 15-Lipoxygenase 1 Antibody (NBP2-01740)

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Recent Reviews

5
5
1
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2
0
1
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Anonymous
09/10/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ALOX15
Entrez