15-Lipoxygenase 1 Antibody (3G8)

Images

 
Western Blot: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - COS7 cells
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Western Blot: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: 15 Lipoxygenase 1 Antibody (3G8) [NBP2-01740] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, Ca, Mk, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

15-Lipoxygenase 1 Antibody (3G8) Summary

Immunogen
Full length human recombinant protein of human 15-Lipoxygenase 1 Antibody (3G8) (NP_001131) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ALOX15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Reviewed Applications
Read 1 Review rated 5
using
NBP2-01740 in the following applications:

Publications
Read Publication using NBP2-01740.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for 15-Lipoxygenase 1 Antibody (3G8)

  • 15-lipooxygenase-1
  • 15-LOX-1
  • 15LOX-1
  • arachidonate 15-lipoxygenase
  • Arachidonate omega-6 lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.33
  • LOG15,15-LOX

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 15-Lipoxygenase 1 Antibody (NBP2-01740)(1)

Review for 15-Lipoxygenase 1 Antibody (NBP2-01740) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-01740:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot 15-Lipoxygenase 1 NBP2-01740
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reviewed by:
Anonymous
WB Human 09/10/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman retinal endothelial cells and U937
SpeciesHuman
LotA001

Blocking

Blocking Details5% non fat Milk for 1 hour at room temp.

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-mouse
Secondary Manufacturer Cat#Cell signaling, 7076s
Secondary Concentration1:5000

Details

Detection NotesX-ray, using supersignal west pico chemiluminescent kit

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 15-Lipoxygenase 1 Antibody (NBP2-01740) (0)

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Secondary Antibodies

 

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Diseases for 15-Lipoxygenase 1 Antibody (NBP2-01740)

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PTMs for 15-Lipoxygenase 1 Antibody (NBP2-01740)

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Recent Reviews

5
5
1
4
0
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2
0
1
0

Anonymous
09/10/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ALOX15
Entrez