14-3-3 zeta/delta Antibody

Images

 
Western Blot: 14-3-3 zeta/delta Antibody [NB100-92268] - Extracts from K562 cells.
Immunohistochemistry-Paraffin: 14-3-3 zeta/delta Antibody [NB100-92268] - Paraffin-embedded human colon carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92268
    • Availability
      Product Discontinued

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14-3-3 zeta/delta Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 zeta.
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAZ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of 14-3-3 Zeta /Delta (W228) pAb in extracts from K562 cells.
Immunohistochemistry (IHC) analyzes of 14-3-3 Zeta /Delta (W228) pAb in paraffin-embedded human colon carcinoma tissue.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
14-3-3 zeta Lysate (NBL1-17952)

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 14-3-3 zeta/delta Antibody

  • KCIP-1MGC126532,14-3-3-zeta
  • MGC111427
  • MGC138156
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
  • zeta polypeptide

Background

14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for 14-3-3 zeta/delta Antibody (NB100-92268)

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Diseases for 14-3-3 zeta/delta Antibody (NB100-92268)

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Bioinformatics

Gene Symbol YWHAZ
Entrez