14-3-3 zeta Antibody (2E11)

Product Discontinued

14-3-3 zeta Antibody (2E11) Summary

• Immunogen: Within the range of amino acids 222-259 of human 14-3-3 zeta were used as the immunogen for the antibody.
• Predicted Species: Porcine (100%), Bovine (100%), Feline (100%), Primate (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAZ
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 1-3ug/ml~

Reactivity Notes

The 14-3-3 zeta amino acid sequence used as immunogen is reactive in human, mouse, and rat. It is predicted to detect bovine, chicken, feline, porcine, most primates based on homology. In human, the immunogen shares 79% with the 14-3-3 alpha/beta protein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for 14-3-3 zeta Antibody (2E11)

• 1433 zeta
• 14-3-3 zeta
• KCIP-1
• KCIP-1MGC126532,14-3-3-zeta
• MGC111427
• MGC138156
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
• YWHAZ
• zeta polypeptide

Background

14-3-3 zeta is a 245 amino acid containing protein belonging to the 14-3-3 family that mediates signal transduction by binding to phosphoserine containing proteins. It is a highly conserved protein in both plants and mammals. The encoded protein interacts with IRS1 implicating a plausible role in regulating insulin sensitivity. There are several transcript variants, with seven known mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta and eta. 14-3-3 proteins function in broad regulation of proteins by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, and activation/repression of enzyme activity. The protein is highly abundant in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 zeta Antibody (NBP2-27179)(3)

Publications using NBP2-27179

• Chauhan SS, Kaur J, Kumar M et al. Prediction of recurrence-free survival using a protein expression-based risk classifier for head and neck cancer Oncogenesis 2015-04-21 [PMID: 25893634] (IHC-P, Human)
• Kaur J, Matta A, Kak I et al. S100A7 overexpression is a predictive marker for high risk of malignant transformation in oral dysplasia. Int. J. Cancer. 2014-03-15 [PMID: 24122701] (IHC-P, Human)
  
  Details:
  Oral lesions, Fig 1, Table 2. 14-3-3 zeta was detected in both the cytoplasm and nucleus.
• Chaker S, Kashat L, Voisin S et al. Secretome proteins as candidate biomarkers for aggressive thyroid carcinomas. Proteomics. 2013-03-01 [PMID: 23319340]
  
  Details:
  The following antibodies cited were used for western blot and IHC:(p) IMG-6664 in thyroid carcinoma and benign thyroid cells. (See Figs 2 and 3).

Bioinformatics

• Gene Symbol: YWHAZ
• Uniprot:
  • P63104()