14-3-3 sigma/Stratifin Antibody

Images

 
Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) ...read more
Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.
Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of ...read more

Product Details

Summary
Reactivity Hu, Am, Bv, Ca, Eq, Op, PmSpecies Glossary
Applications WB, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

14-3-3 sigma/Stratifin Antibody Summary

Immunogen
A portion of amino acids 120-170 of human 14-3-3 sigma was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFN
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 3-5ug/ml~
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10ug/ml
  • Knockdown Validated
Control
14-3-3 sigma/Stratifin Knockout HeLa Cell Lysate

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in human, mouse, rat, pig, new world monkey, panda, cow, dog, orangutan, rhesus monkey, white-cheeked gibbon, chimpanzee, rabbit, and sheep, 94% in horse, 89% in opossum, and 72% in lizard.This antibody will detect both isoforms of 14-3-3 sigma (248 amino acids and 169 amino acids).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for 14-3-3 sigma/Stratifin Antibody

  • 14-3-3 protein sigma
  • 1433 sigma
  • 14-3-3 sigma
  • Epithelial cell marker protein 1
  • HME1
  • SFN
  • stratifin
  • YWHAS

Background

14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms (sigma epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigma is the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to irradiation and other DNA-damaging agents. 14-3-3 sigma is a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SFN
Uniprot