14-3-3 sigma/Stratifin Antibody

Images

 
Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-81672] - Detection of Human 14-3-3 Sigma by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from Hela cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry: 14-3-3 sigma/Stratifin Antibody [NB100-81672] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- 14-3-3 Sigma used at a dilution of 1:1,000 (1ug/ml). ...read more
Proximity Ligation Assay: 14-3-3 sigma/Stratifin Antibody [NB100-81672] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human 14-3 Sigma (NB100-81672). Image merged ...read more
Proximity Ligation Assay: 14-3-3 sigma/Stratifin Antibody [NB100-81672] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human 14-3 Sigma (NB100-81672). Image ...read more
Proximity Ligation Assay: 14-3-3 sigma/Stratifin Antibody [NB100-81672] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HdmX (NBP2-22228) and rabbit anti-human 14-3 Sigma (NB100-81672). Image merged ...read more
Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NB100-81672] - Detection of Human 14-3-3 Sigma on HeLa whole cell lysate using NB100-81672. 14-3-3 sigma/Stratifin was also immunoprecipitated by another rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

14-3-3 sigma/Stratifin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 198 and 248 of human 14-3-3 sigma/Stratifin using the numbering given in entry NP_006133.1 (GeneID 2810).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Positive Control
A-431 Lysate (NB820-59461)
Bladder Lysate (NB820-59173)
14-3-3 sigma/Stratifin Lysate (NBL1-15882)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 14-3-3 sigma/Stratifin Antibody

  • 14-3-3 protein sigma
  • 1433 sigma
  • 14-3-3 sigma
  • Epithelial cell marker protein 1
  • HME1
  • SFN
  • stratifin
  • YWHAS

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SFN
Entrez
Uniprot