14-3-3 gamma Antibody

Product Discontinued

14-3-3 gamma Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human 14-3-3 gamma.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 14-3-3 gamma Antibody

• 1433 gamma
• 14-3-3 gamma
• 14-3-3 protein gamma
• 14-3-3GAMMA
• KCIP-1
• Protein kinase C inhibitor protein 1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide
• YWHAG

Background

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, episilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAG
• Entrez:
  • 7532(Human)
  • 22628(Mouse)
  • 56010(Rat)
• Uniprot:
  • P61981()