14-3-3 eta Antibody [Unconjugated]

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3 eta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 eta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

14-3-3 eta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human 14‑3‑3 eta
  Met1-Asn246
  Accession # Q04917
• Specificity: Detects human, mouse, and rat 14-3-3 eta in Western blots. In Western Blot less than 1% cross reactivity with other recombinant human 14-3-3 isoforms.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: YWHAH
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 14-3-3 eta Antibody [Unconjugated]

• 1433 eta
• 14-3-3 eta
• 14-3-3 protein eta
• Protein AS1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
• YWHA1
• YWHA114-3-3 eta
• YWHAH

Background

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta , epsilon , eta , gamma , tau / theta , sigma , and zeta /δ.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 eta Antibody (AF4420)(1)

Publication using AF4420

• AA Eisa, S De, A Detwiler, E Gilker, AC Ignatious, S Vijayaragh, D Kline YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation BMC Dev. Biol., 2019-10-22;19(1):20. 2019-10-22 [PMID: 31640562] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: YWHAH
• Uniprot:
  • Q04917()