14-3-3 epsilon Antibody (5A5)

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Western Blot: 14-3-3 epsilon Antibody (5A5) [NBP1-22960] The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 epsilon (1:1000). Proteins were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PAGE
Clone
5A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

14-3-3 epsilon Antibody (5A5) Summary

Immunogen
Recombinant human 14-3-3 epsilon (1-255aa) purified from E. coli
Specificity
Anti-human 14-3-3 epsilon mAb, clone 5A5, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-3 epsilon protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
YWHAE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:100-1:2000
  • SDS-Page 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for 14-3-3 epsilon Antibody (5A5)

  • 1433 epsilon
  • 14-3-3 epsilon
  • 14-3-3 protein epsilon
  • FLJ45465
  • FLJ53559,14-3-3E
  • KCIP-1
  • MDCR
  • MDS
  • mitochondrial import stimulation factor L subunit
  • protein kinase C inhibitor protein-1
  • tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide
  • YWHAE

Background

This protein belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 proteins are localized in neurons, and are axonally transported to the nerve terminals. They may be also present, at lower levels, in various other eukaryotic tissues. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol YWHAE
Entrez
Uniprot