14-3-3 beta/alpha Antibody

Product Discontinued

14-3-3 beta/alpha Antibody Summary

• Immunogen: A synthetic peptide corresponding to N-term of human 14.3.3 beta protein.
• Specificity: This antibody recognizes ~28 kDa of human 14.3.3 beta. It does not react to the other isoforms. This antibody reacts with human, rat and mouse. The other species are not tested.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.01-0.1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 2-5 ug/ml
  • Western Blot 1-2 ug/ml

Reactivity Notes

Reactive in Human, Mouse, and Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile TBS (pH 7.2) with antibody stabilizer
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 14-3-3 beta/alpha Antibody

• 14-3-3 alpha
• 14-3-3 beta
• 14-3-3 protein beta/alpha
• brain protein 14-3-3, beta isoform
• GW128
• HS1
• KCIP-1
• Protein 1054
• Protein kinase C inhibitor protein 1
• protein kinase C inhibitor protein-1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
• YWHAA

Background

The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14.3.3 beta is highly expressed in brain tissue. It is critical to cell transformation and mitotic signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 beta/alpha Antibody (NBP1-72185)(1)

Publication using NBP1-72185

• Luo ZJ, Zhang XF, Rapp U, Avruch J Diabetes Research Laboratory, Massachusetts General Hospital, Charlestown 02129, USA. Identification of the 14.3.3 zeta domains important for self-association and Raf binding. J Biol Chem. 270(40):23681-7. 1995-10-06 [PMID: 7559537]

Bioinformatics

• Gene Symbol: YWHAB