14-3-3 beta/alpha Antibody

Images

 
Western Blot: 14-3-3 beta/alpha Antibody [NB100-92414] - Extracts from HepG2 cells.
Western Blot: 14-3-3 beta/alpha Antibody [NB100-92414] - Western blot (WB) analysis of 14-3-3 beta (Q69) pAb in extracts from THP1 cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92414
    • Availability
      Product Discontinued

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14-3-3 beta/alpha Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 40-90 of Human 14-3-3 beta.
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of 14-3-3 Beta (Q69) pAb in extracts from THP1 cells.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
14-3-3 tau/theta Lysate (NBL1-17951)

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 14-3-3 beta/alpha Antibody

  • 14-3-3 alpha
  • 14-3-3 beta
  • 14-3-3 protein beta/alpha
  • brain protein 14-3-3, beta isoform
  • GW128
  • HS1
  • KCIP-1
  • Protein 1054
  • Protein kinase C inhibitor protein 1
  • protein kinase C inhibitor protein-1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
  • YWHAA

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YWHAB
Entrez