14-3-3 beta/alpha [p Ser58] Antibody

Product Discontinued

14-3-3 beta/alpha [p Ser58] Antibody Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
• Modification: p Ser58
• Specificity: Specific for the ~29k 14-3-3 beta/alpha protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Application Notes: This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~29 kDa. No other applications have been tested. *The investigator should determine the optimal working dilution for a specific application.
• Control:

  14-3-3 tau/theta Overexpression Lysate

Reactivity Notes

Tested in Human and Rat. Reactivity assumed based on 100% sequence homology: Bovine, Canine, Chicken, Mouse, Primate, Sheep, Xenopus and Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for 14-3-3 beta/alpha [p Ser58] Antibody

• 14-3-3 alpha
• 14-3-3 beta
• 14-3-3 protein beta/alpha
• brain protein 14-3-3, beta isoform
• GW128
• HS1
• KCIP-1
• Protein 1054
• Protein kinase C inhibitor protein 1
• protein kinase C inhibitor protein-1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
• YWHAA

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 beta/alpha Antibody (NB100-668)(1)

Publication using NB100-668

• Korsholm LM, Gal Z, Lin L et al. Double-strand breaks in ribosomal RNA genes activate a distinct signaling and chromatin response to facilitate nucleolar restructuring and repair Nucleic Acids Res. 2019-06-11 [PMID: 31184714] (ICC/IF, WB, Human)
  
  Details:
  Induction of double strand breaks in human ribosomal RNA genes in the nucleolus is utilized to highlight the roles of the nucleolar DNA damage response.

Bioinformatics

• Gene Symbol: YWHAB
• Entrez:
  • 7529(Human)
• Uniprot:
  • P35213(Rat)