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![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0004.jpg "Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.")
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase reporter gene under the transcriptional control of an NF-KB response element. IML-102 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: specifically activated the TLR2/1-depedent NF-KB/SEAP reporter cells in a dose dependent manner (Note: HEK 293 cells endogenously express TLR1).](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0005.jpg)
![Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit](http://images.novusbio.com/fullsize2/Polyinosinic-polycytidylic-acid-NBP2-25288-img0002.jpg "Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit")
![Imiquimod, TLR7 ligand [NBP2-26228] - 293T cells were transfected with pCMV/TLR7 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 5 ug/mL of Imiquimod (R-837) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorter Assay kit.](http://images.novusbio.com/fullsize2/Imiquimod--TLR7-ligand-NBP2-26228-img0002.jpg "Imiquimod, TLR7 ligand [NBP2-26228] - 293T cells were transfected with pCMV/TLR7 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 5 ug/mL of Imiquimod (R-837) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorter Assay kit.")
![Zymosan, TLR2 Ligand [NBP2-26233] - Ligand activity of Zymosan. The NF-kB/SEAPorter/HEK 293 and TLR2/NFkB/SEAPorter/ HEK 293 cell line cell lines were plated in a 96-well plate at 5 x 10^4 cells/well for 16 h. Cells were then stimulated with Zymosan for 24h. The cell culture supernatants were analyzed for SEAP activity using SEAPorter Assay Kit.](http://images.novusbio.com/fullsize2/Zymosan--TLR2-Ligand-NBP2-26233-img0002.jpg "Zymosan, TLR2 Ligand [NBP2-26233] - Ligand activity of Zymosan. The NF-kB/SEAPorter/HEK 293 and TLR2/NFkB/SEAPorter/ HEK 293 cell line cell lines were plated in a 96-well plate at 5 x 10^4 cells/well for 16 h. Cells were then stimulated with Zymosan for 24h. The cell culture supernatants were analyzed for SEAP activity using SEAPorter Assay Kit.")
![CpG oligodeoxynucleotides Mouse [NBP2-26235] - CpG oligodeoxynucleotides Mouse Evaluation of the mCpG ODN 1826 ligand activity on NF-kB SEAPorter RAW cell line. Cell line is a stably transfected RAW 264.7 cell line that expresses the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. RAW 264.7 cells endogenously express Toll-like receptor 9 (TLR9). Cells were plated in 96-well plates at 0.85 x 10^5 cells/well. After 16 h, cells were stimulated with various amounts of mCpG ODN 1826 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: mCpG ODN 1826 specifically activated the TLR9-depedent NF-kB/SEAP reporter cells in a dose dependent manner.](http://images.novusbio.com/fullsize2/CpG-oligodeoxynucleotides-Mouse-NBP2-26235-img0002.jpg "CpG oligodeoxynucleotides Mouse [NBP2-26235] - CpG oligodeoxynucleotides Mouse Evaluation of the mCpG ODN 1826 ligand activity on NF-kB SEAPorter RAW cell line. Cell line is a stably transfected RAW 264.7 cell line that expresses the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. RAW 264.7 cells endogenously express Toll-like receptor 9 (TLR9). Cells were plated in 96-well plates at 0.85 x 10^5 cells/well. After 16 h, cells were stimulated with various amounts of mCpG ODN 1826 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: mCpG ODN 1826 specifically activated the TLR9-depedent NF-kB/SEAP reporter cells in a dose dependent manner.")
![CpG oligodeoxynucleotides [NBP2-26238]](http://images.novusbio.com/fullsize2/CpG-oligodeoxynucleotides-NBP2-26238-img0001.jpg "CpG oligodeoxynucleotides [NBP2-26238]")
![CpG oligodeoxynucleotides [NBP2-26238] - Evaluation of various CpG ODN effects on activation of human TLR9. TLR9/NF-kB/SEAPorter HEK 293 cells were plated in 96-well plates at 7.5 x 10^4 cells/well for 16 h. Cells were treated with various CpG ODNs at different concentrations (1, 5, 10 and 20 ug/ml) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit. Note: Both negative hCpG and mCpG are the negative control ODNs.](http://images.novusbio.com/fullsize2/CpG-oligodeoxynucleotides-NBP2-26238-img0002.jpg)