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Novus provides a variety of support products for research assays. These products include antigen retrieval buffers, knockout stem cells, g-linked agarose resins, purified human sera, and PBS tablets.
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![pCL-Eco Retrovirus Packaging Vector [NBP2-29540]](http://images.novusbio.com/fullsize2/pCLNDX-Retrovirus-Expression-Vector-NBP2-29540-img0001.jpg "pCL-Eco Retrovirus Packaging Vector [NBP2-29540]")
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0001.jpg "PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0002.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0008.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0007.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0003.jpg "PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0004.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0005.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0006.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the mesoderm marker, Smooth Muscle Actin, was detected in 15 day cells using Mouse Anti-Smooth Muscle Actin monoclonal antibody (R&D Systems, Catalog # MAB1420). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0013.jpg "Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the mesoderm marker, Smooth Muscle Actin, was detected in 15 day cells using Mouse Anti-Smooth Muscle Actin monoclonal antibody (R&D Systems, Catalog # MAB1420). The nuclei were counterstained with DAPI.")
![Flow Cytometry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker SSEA-1 and Lack SSEA-4. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Flow-Cytometry-NBP1-41162-img0014.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker Oct-3/4. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of Oct-3/4 was detected using Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (R&D Systems, Catalog # MAB1759) followed by NorthernLights (NL)557-conjugated Goat Anti-Rat Secondary Antibody (R&D Systems, Catalog # NL013). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0009.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker Alkaline Phosphatase. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of Alkaline Phosphatase was detected using Goat Anti-Mouse Alkaline Phosphatase Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF2910) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody (R&D Systems, Catalog # NL001). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0010.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the ectoderm marker, SOX1, was detected in day 10 cells using Goat Anti-SOX1 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF3369) followed by NorthernLights (NL)557-conjugated Donkey Anti-Goat Secondary Antibody. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0011.jpg)
![In vitro assay: v6.5 Mouse embryonic stem cells [NBP1-41162] - Brightfield Image of v6.5 Mouse embryonic stem cell [NBP1-41162] colonies growing on a Mitomycin C treated MEF cell feeder layer.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-In-vitro-assay-NBP1-41162-img0003.jpg)
![In vitro assay: v6.5 Mouse embryonic stem cells [NBP1-41162] - Brightfield Image of v6.5 Mouse embryonic stem cell [NBP1-41162] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-In-vitro-assay-NBP1-41162-img0004.jpg)
![Immunodiffusion: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the endoderm marker, SOX17, was detected in 10 day cells using Goat Anti-SOX17 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF1924) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunodiffusion-NBP1-41162-img0012.jpg)
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0004.jpg "Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.")
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase reporter gene under the transcriptional control of an NF-KB response element. IML-102 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of for 24 h. SEAP was analyzed using the SEAPorter Assay Kit . Data Summary: specifically activated the TLR2/1-depedent NF-KB/SEAP reporter cells in a dose dependent manner (Note: HEK 293 cells endogenously express TLR1).](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0005.jpg)
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Differentiated human macrophages were treated with or without 50 ng/mL Pam3CSK4 for 2 h, and the release of IL-6 in culture supernatants of cells was measured by ELISA kit.](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0006.jpg)
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.](http://images.novusbio.com/fullsize2/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0003.jpg "MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.")
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Evaluation of the MALP2 ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line.Cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. Cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of MALP2 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: MALP2 specifically activated the TLR2/6-depedent NF-kB/SEAP reporter cells in a dose dependent manner. Note: HEK 293 cells endogenously express TLR6.](http://images.novusbio.com/fullsize2/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0004.jpg)
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Differentiated human macrophages were treated with or without MALP-2 (100 ng/mL), and the release of IL-6 in culture supernatants of cells was measured by ELISA kit. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.](http://images.novusbio.com/fullsize2/DAPI-Solution-Immunofluorescence-NBP2-31156-img0002.jpg "Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.")
![Immunohistochemistry: DAPI Solution [NBP2-31156] - DAPI labelling in mouse brain. Image from verified customer review.](http://images.novusbio.com/fullsize2/DAPI-Solution-Immunohistochemistry-NBP2-31156-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize2/DAPI-Solution-Immunocytochemistry-Immunofluorescence-NBP2-31156-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0008.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025). At passage 5, cells were harvested and analyzed for pluripotent markers by flow cytometry. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A). Quadrant lines were drawn based on matched isotype controls.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-Flow-Cytometry-NBA1-19349-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Nanog antibody (NB100-58842) was tested in with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0007.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0009.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing on a Mitomycin C MEF cell feeder layer.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0010.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0011.jpg)
![Imiquimod, TLR7 ligand [NBP2-26228] - 293T cells were transfected with pCMV/TLR7 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 5 ug/mL of Imiquimod (R-837) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorter Assay kit.](http://images.novusbio.com/fullsize2/Imiquimod--TLR7-ligand-NBP2-26228-img0002.jpg "Imiquimod, TLR7 ligand [NBP2-26228] - 293T cells were transfected with pCMV/TLR7 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 5 ug/mL of Imiquimod (R-837) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorter Assay kit.")