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| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33244 | |||
![Knockout Validated: LC3B Antibody [NB100-2220] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Knockout-Validated-NB100-2220-img0045.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody [NB100-2220] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody [NB100-2220] - LC3B/MAP1 [NB100-2220] - Detection of LC3 in mouse ES cell lysates. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT SW Medical Center.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Knockout-Validated-NB100-2220-img0047.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody [NB100-2220] - LC3B/MAP1 [NB100-2220] - Western blot analysis of HeLa (1), HeLa + CQ (2), SHSY5Y (3), SHSY5Y +CQ (4), A431 (5), A431 +CQ (6) and Ntera2 (7) using LC3 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0034.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody [NB100-2220] - LC3 was detected in immersion fixed Cloroquine treated Hela cells (left) but was not detected in LC3 knockout Hela cells (right) using rabbit anti-human LC3 polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Knockout-Validated-NB100-2220-img0059.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody [NB100-2220] - shows lysates of mouse NIH3T3 and rat PC-12 cell lines untreated (-) or treated (+) with Chloroquine. PVDF membrane was probed with 0.5 ug/mL rabbit anti-LC3B polyclonal Antibody (NB100-2220, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG secondary antibody.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0031.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Analysis of LC3 in Huh-7 and SMMC-7721 cells using anti-LC3B antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0028.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Analysis using the Biotin conjugate of NB100-2220. Image from verified customer review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0040.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody [NB100-2220] - Human ovarian Cancer tissue stained using heat mediated antigen retrieval in pH 6.0 citrate buffer at 1:200 dilution. Image provided by verified customer review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB100-2220] - LC3B detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Chloroquine using 1ug/mL rabbit anti-LC3B polyclonal (NB100-2220, Novus Biologicals). Cells were stained using donkey anti-rabbit IgG-NL557 and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0036.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Autophagy Investigation. (A) Representative WBs of LC3B-I, LC3B-II and GAPDH loading control in DANs derived from 4 iPD patients and 4 healthy controls. (B) WBs from three independent experiments were quantified by densitometry and LC3B-II normalized to a loading control. Grouping of data by healthy vs iPD as well as iPD stratified for T/T and C/C genotype. (C) Ratio of LC3B-II (normalized to loading control) following bafilomycin treatment/untreated. Citation: Marrone L, Bus C, Schondorf D, Fitzgerald JC, Kubler M, Schmid B, et al. (2018) Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease. PLoS ONE 13(3): e0192497. //doi.org/10.1371/journal.pone.0192497](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0061.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB100-2220] - LC3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0009.jpg)
![Immunohistochemistry: LC3B Antibody [NB100-2220] - Analysis using the Biotin conjugate of NB100-2220. Staining of brain, cerebral cortex, neurons with cell processes.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0053.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Analysis of LC3 in H9C2 cells.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0035.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Analysis using the DyLight 550 conjugate of NB100-2220. Detection of LC3 in pancreatic cancer cells.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0041.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Detection of LC3 in mouse HL-1 cell lysate. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody [NB100-2220] - WB detection of LC3I and LC3II in mouse cochlea cell line SV-K1. Cells were treated with chloroquine (1uM), and As2O3 (1uM) for 24 hrs. This image was submitted via customer review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0056.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Detection in fibroblast whole cell extract. Image from verified customer review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0058.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Macrophage lysates probed with anti-LC3 antibody. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0052.jpg)
![Western Blot: LC3B Antibody [NB100-2220] - Uncropped western blots used for quantification. Detection of LC3B. Normalized Protein levels by the indicated housekeeping protein. iPD genotypes are reported. Blots show protein levels in the presence or absence of a specified treatment. Baf = bafilomycin, Rot = rotenone, Val = valinomycin, CZC = CZC-25146Citation: Marrone L, Bus C, Schondorf D, Fitzgerald JC, Kubler M, Schmid B, et al. (2018) Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease. PLoS ONE 13(3): e0192497. //doi.org/10.1371/journal.pone.0192497](http://images.novusbio.com/fullsize2/LC3B-Antibody-Western-Blot-NB100-2220-img0060.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB100-2220] - HeLa cells were treated with 50uM CQ overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LC3B at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0049.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB100-2220] - Untreated HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LC3B at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2220-img0050.jpg)
![Immunohistochemistry: LC3B Antibody [NB100-2220] - IHC of rat brain from confirmed customer review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0037.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody [NB100-2220] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse brain using 1:200 dilution of rabbit anti-LC3 antibody. The specific signal of LC3 was detected using HRP-conjugated secondary antibody with DAB reagent, and nuclei of cells were counterstained using hematoxylin. This LC3 antibody generated a low to moderate levels of cytoplasmic staining in the glial cells. The neurons depicted a moderate to strong staining for LC3 in their cytoplasm.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0048.jpg)
![Immunohistochemistry-Frozen: LC3B Antibody [NB100-2220] - LC3 accumulation in muscle fibers from patients with PAD (row B - E). Normal fibers from a Non-PAD sample (row A). Scale bar = 100 uM. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Immunohistochemistry-Frozen-NB100-2220-img0051.jpg)
![Simple Western: LC3B Antibody [NB100-2220] - Simple Western lane view shows a specific band for LC3 in 0.5 mg/ml of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/LC3B-Antibody-Simple-Western-NB100-2220-img0016.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Knockout-Validated-NB100-105-img0027.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0022.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0021.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-NB100-105-img0004.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Biotin conjugate of NB100-105. Staining of human glioblastoma multiforme.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-Paraffin-NB100-105-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Expression of HIF-1 in intresititial pig cells under 12% of 02 condition. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunocytochemistry-Immunofluorescence-NB100-105-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1a was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA using 1 ug/mL of mouse anti-HIF-1a monoclonal (NB100-105, Novus Biologicals). Cells were stained using donkey anti-rabbit and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunocytochemistry-Immunofluorescence-NB100-105-img0023.jpg)
![Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Flow-Cytometry-NB100-105-img0016.jpg)
![Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1a antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Flow-Cytometry-NB100-105-img0017.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Figure Legend: Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1a-deficient (Hif1a KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1a and Lamin B1 levels were analyzed by Western blot. This image was submitted via customer Review. (Mouse, WB)](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0026.jpg)
![Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - 786-O cells without or with VHL overexpression. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - 786-O cells without or with VHL overexpression. Image from verified customer review.")
![<b>Independent Antibodies Validation, Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-2 alpha in MDA-MB-231 cell lysate (overexpression and endogenous samples) using anti-HIF-2 alpha antibody. HIF-2 alpha antibody did not react to HIF-1 alpha overexpression. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-2 alpha antibody was tested in RCC4 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-122-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-2 alpha in human retinal and choroidal primary endothelia lysates using anti-HIF-2 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0022.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis using the HRP conjugate of NB100-122. Detection of normoxic and hypoxic nuclear rat cell lysates.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0021.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Lane 1: Cobalt chloride treated COS7 nuclear extracts. Lane 2: Untreated COS7 nuclear extracts.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0010.jpg)
![Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF2 expression in HIF2 over-expressed HEK 293T cells. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0023.jpg)
![Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Expression and correlation of 3 antibodies: HIF-1 alpha, HIF-2 alpha and HAF in ccRCC. (A, C & E) Positive nuclear staining to the 3 antibodies: HIF-1 alpha, HIF-2 alpha and HAF, in primary ccRCCs at high magnification (x100). (B, D & F) Heterogenous nuclear staining of 3 antibodies in a tumor at low magnification (x20). (G, H and I) Correlation of 3 antibodies with the Fuhrman grade.Citation: Ambrosetti D, Dufies M, Dadone B, Durand M, Borchiellini D, Amiel J, et al. (2018) The two glycolytic markers GLUT1 and MCT1 correlate with tumor grade and survival in clear-cell renal cell carcinoma. PLoS ONE 13(2): e0193477. //doi.org/10.1371/journal.pone.0193477](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Immunohistochemistry-NB100-122-img0027.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis on normoxic and hypoxic nuclear rat cell lysates.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0008.jpg)
![Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Human Glioma whole cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Western-Blot-NB100-122-img0011.jpg)
![Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Hif-2 alpha immunoreactivity in human cardiac myocytes stained with NB100-122.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Immunohistochemistry-Paraffin-NB100-122-img0020.jpg)
![Simple Western: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Image shows a specific band for HIF-2 alpha in 0.5 mg/mL of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Simple-Western-NB100-122-img0013.jpg)
![Immunoblotting: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of protein extracts by immunoblotting against the N-terminus of VDAC1 isoform (VDAC1-Nter), the three VDAC isoforms (VDACs poly), ISCU and CAIX/ HIF-2 alpha/EPAS1 antibody. Citation: Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen M-P (2018) Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1 alpha activation. PLoS ONE 13(3): e0194782.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Immunoblotting-NB100-122-img0026.jpg)
![Immunoblotting: HIF-2 alpha/EPAS1 Antibody [NB100-122] - CAIX/ HIF-2 alpha/EPAS1 and other antibodies used for immunoblotting to analyze the hypoxia (Hx, 1% O2) or total protein extracts from HeLa cells grown in normoxia (Nx, 21% O2) conditions. Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen M-P (2018) Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1 alpha activation. PLoS ONE 13(3): e0194782.](http://images.novusbio.com/fullsize2/HIF-2-alpha-EPAS1-Antibody-Immunoblotting-NB100-122-img0025.jpg)
![Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-53BP1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Western-Blot-NB100-304-img0029.jpg "Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-53BP1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Knockout-Validated-NB100-304-img0031.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunohistochemistry-Frozen-NB100-304-img0015.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - 53BP1 foci in proliferating MEFs; both normal and exposed to 10 Gy of IR.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0019.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Detection of Human and Mouse 53BP1 by IHC. Sample: FFPE sections of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: NB100-304 used at a dilution of 1:1000 (1ug/mL). Detection: DAB.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunohistochemistry-Paraffin-NB100-304-img0013.jpg)
![Flow Cytometry: 53BP1 Antibody [NB100-304] - 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 ug/ml anti-53BP1 NB100-304 in a 150 uL reaction. Isotype control (black), anti-53BP1 (red).](http://images.novusbio.com/fullsize2/53BP1-Antibody-Flow-Cytometry-NB100-304-img0021.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Human breast tumors stained with 53BP1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunohistochemistry-Paraffin-NB100-304-img0017.jpg)
![Western Blot: 53BP1 Antibody [NB100-304] - Whole cell lysate from U2OS or 293T cells.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Western-Blot-NB100-304-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: Control untreated cells. Lower Panel: Cells exposed to irradiation (10 Gy) and probed for 53BP1 foci. Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with primary antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa Fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil). Image from verified customer review.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0027.jpg)
![Flow (Intracellular): 53BP1 Antibody [NB100-304] - An intracellular stain was performed on Neuro2A cells with NB100-304G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Flow-Cytometry-NB100-304-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - 53BP1 antibody was tested at 1:100 in HeLa cells with FITC (green). Alpha-Tubulin antibody NB100-690 was tested at 1:500 using Mouse IgG secondary antibody NB710-94914 at 1:1000 [HiLyte Fluor 555] (red). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 1 ug/mL of rabbit anti- 53BP1 polyclonal (NB100-304, Novus Biologicals). Cells were stained donkey anti-goat IgGNL557 and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 at 2 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 at 2 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 at 2 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-53BP1 conjugated to DyLight 550 [NB100-304R] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0028.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-53BP1 conjugated to Alexa Fluor 488 [NB100-304AF488] at 10 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent Etoposide.Cells were immunostained for 53BP1 (green). The nuclei were counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Embryonic Fibroblast cells pre-extraction for 5 mins with CSK buffer. Fixed with 4% PFA and 75% Ethanol. Primary Antibody at 1:1000. Secondary Antibody at 1:1000. Image from verified customer review.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-304-img0026.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Staining of 53BP1 in human colon cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Immunohistochemistry-Paraffin-NB100-304-img0014.jpg)
![Flow (Intracellular): 53BP1 Antibody [NB100-304] - An intracellular stain was performed on Neuro2A cells with NB100-304F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize2/53BP1-Antibody-Flow-Cytometry-NB100-304-img0032.jpg)
![Knockout Validated: Nbs1 Antibody [NB100-143] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Nbs1 Polyclonal Antibody (Catalog # NB100-143) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/Nbs1-Antibody-Knockout-Validated-NB100-143-img0015.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Nbs1 Antibody [NB100-143] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Nbs1 Polyclonal Antibody (Catalog # NB100-143) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody [NB100-143] - TIG-1 human primary fibroblasts, whole cell lysate (30 ug). Image from verified customer review.](http://images.novusbio.com/fullsize2/Nbs1-Antibody-Western-Blot-NB100-143-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - Hela cells. Image from verified customer review.](http://images.novusbio.com/fullsize2/Nbs1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-143-img0011.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-143] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human testis using Nbs1 antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize2/Nbs1-Antibody-Immunohistochemistry-Paraffin-NB100-143-img0014.jpg)
![Western Blot: Nbs1 Antibody [NB100-143] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.](http://images.novusbio.com/fullsize2/Nbs1-Antibody-Western-Blot-NB100-143-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody [NB100-143] - Detection of NBS1 in Human Bone Osteosarcoma Epithelial Cells (U2OS whole cell lysate) using NB100-143 at a dilution of 1/1000. Specificity was confirmed using a siRNA anti-NBS1 (targeting sequence: GGAAGAAACGUGAACUCAA). Image provided by Sebastien Britton of the Institute of Pharmacology and Structural Biology.](http://images.novusbio.com/fullsize2/Nbs1-Antibody-Western-Blot-NB100-143-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - Staining of Hela cells using NB100-143 anti-NBS1.](http://images.novusbio.com/fullsize2/Nbs1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-143-img0009.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in overexpression and endogenous HIF1 alpha & HIF2 alpha using anti-HIF-1 alpha antibody. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0011.jpg "<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in overexpression and endogenous HIF1 alpha & HIF2 alpha using anti-HIF-1 alpha antibody. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Image from verified customer review.")
![<b>Independent Antibodies Validation, Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of NB100-134, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0007.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: HIF-1 alpha Antibody [NB100-134] - PC-3 Cell Lysates run with Cobalt Chloride treatments. Immunoprecipitation was performed with protein A/G Agarose beads. This data was provided courtesy of Kelie Reece, Figg lab, NCI.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunoprecipitation-NB100-134-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in ARPE-19 cells using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-134-img0010.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Staining in the canine CL on days 10 to 70 after ovulation. PC = positive control (human placenta). NC = negative control. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-134-img0008.jpg)
![Simple Western: HIF-1 alpha Antibody [NB100-134] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Simple-Western-NB100-134-img0009.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Analysis of FFPE tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF1 alpha antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-134-img0012.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha on Zebrafish retina tissue using HIF-1 alpha antibody. Mouse tissue was used as a control. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-134-img0014.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-134] - 20 ug whole cell lysates (human ovarian cancer cell lines). Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in human Retinal and Choroidal primary endothelia cells using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0013.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody [NB100-134] - Staining of human kidney, renal tubular epithelium in Cortex.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-134-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-123.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-123-img0023.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-123.")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-123-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining in pig endothelial cells under hypoxia condition. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunocytochemistry-Immunofluorescence-NB100-123-img0011.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis of HIF-1 alpha in human lung tissue. Image courtesy of product review by Aneta Gandjeva.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-NB100-123-img0006.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining of Biotin conjugated HIF-1 alpha in human kidney.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-NB100-123-img0026.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunoprecipitation of HIF-1alpha using NB100-123. Heavy and light chains are also detected. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-123-img0025.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining on pig tissue (brown). Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-Paraffin-NB100-123-img0012.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0032.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3.")
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0031.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0037.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with VP-16. PVDF membrane was probed with 0.1 ug/mL of mouse anti-Caspase-3 monoclonal (NB100-56708, Novus Biologicals) followed by 1:2000 dilution donkey anti-mouse IgG.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0028.jpg)
![Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase 3 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Simple-Western-NB100-56708-img0033.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0042.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using Azide/BSA FREE version of NB100-56708. Analysis of Caspase-3 in A) RAW and B) NIH 3T3 using 32 ug/ml.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0040.jpg)
![Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Flow-Cytometry-NB100-56708-img0043.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Lanes 1, 2 and 3 demonstrate the species cross-reactivity of the antibody in human, mouse and rat heart lysate, respectively.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0038.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse anti-Caspase-3 monoclonal (NB100-56708, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0027.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of human breast cancer stained at 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0029.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the azide free version of NB100-56708. Staining of normal colon stained 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0034.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of the human thymus after heat-induced antigen retrieval at 5 ug/ml.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0035.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: C2C12 10% SDS PAGE diluted at 1:500 Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0021.jpg "Western Blot: TSG101 Antibody (4A10) [NB200-112] - Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: C2C12 10% SDS PAGE diluted at 1:500 Image using the Biotin form of this antibody.")
![Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Paraffin-embedded Colon carcinoma, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Immunohistochemistry-Paraffin-NB200-112-img0006.jpg)
![Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on HeLa cells with TSG101 Antibody (4A10) NB200-112F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Flow-Cytometry-NB200-112-img0022.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0009.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0012.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The signal was developed with Trident Sharp-ECL.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0013.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0014.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0016.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0018.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0007.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents. Image from verified customer review.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-Western-Blot-NB200-112-img0020.jpg)
![ELISA: TSG101 Antibody (4A10) [NB200-112] - ELISA detection of TSG101 for capture at a concentration of 5 ug/mL and TSG101 for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize2/TSG101-Antibody-(4A10)-ELISA-NB200-112-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-2219] - Analysis of EGLN1/PHD2 in ARPE-19 cells using anti-PHD2 antibody. Image from veriifed customer review.](http://images.novusbio.com/fullsize2/EGLN1-PHD2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2219-img0009.jpg "Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-2219] - Analysis of EGLN1/PHD2 in ARPE-19 cells using anti-PHD2 antibody. Image from veriifed customer review.")
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody [NB100-2219] - IHC analysis of a formalin fixed and paraffin embedded mouse lung section using 1:200 dilution of EGLN1/PHD2 antibody. The staining was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. This PHD2 antibody generated a specific staining in the cytoplasm and nuclei of alveolar as well as bronchiolar epithelial cells. Cytoplasmic staining was observed in almost all cells while the nuclear positivity was seen in a subset of cells only.](http://images.novusbio.com/fullsize2/EGLN1-PHD2-Antibody-Immunohistochemistry-Paraffin-NB100-2219-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-2219] - EGLN1/PHD2 antibody (1:500) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/EGLN1-PHD2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2219-img0012.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-2219] - Detection of EGLN1/PHD2 in mouse kidney lysate using NB 100-2219. ECL exposure, 20 seconds.](http://images.novusbio.com/fullsize2/EGLN1-PHD2-Antibody-Western-Blot-NB100-2219-img0013.jpg)
![Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - Staining of renal tubular epithelium in mouse using NB100-2219 at 2.5ug/ml. Mouse kidney cortex, 40X.](http://images.novusbio.com/fullsize2/EGLN1-PHD2-Antibody-Immunohistochemistry-NB100-2219-img0010.jpg)
![Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/Niemann-Pick-C1-Antibody-Knockout-Validated-NB400-148-img0014.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Hela lysate, BHK lysate, A431 lysate tested at 1:1000. Image provided by verified customer review.](http://images.novusbio.com/fullsize2/Niemann-Pick-C1-Antibody-Western-Blot-NB400-148-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody [NB400-148] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Niemann-Pick-C1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-148-img0013.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.](http://images.novusbio.com/fullsize2/Niemann-Pick-C1-Antibody-Immunohistochemistry-Paraffin-NB400-148-img0011.jpg)
![Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Detection of human NPC1 in 20 ug of human fibroblast cell lysate.](http://images.novusbio.com/fullsize2/Niemann-Pick C1 Antibody-Western Blot-NB400-148-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody [NB400-148] - NPC1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize2/Niemann-Pick-C1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-148-img0010.jpg)
![Knockout Validated: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/IkB-alpha-Antibody-(6A920)-Knockout-Validated-NB100-56507-img0032.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).](http://images.novusbio.com/fullsize2/IkB-alpha-Antibody-(6A920)-Western-Blot-NB100-56507-img0031.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [NB100-56507] - Human breast probed with IkBa antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min..](http://images.novusbio.com/fullsize2/IkB-alpha-Antibody-(6A920)-Immunohistochemistry-Paraffin-NB100-56507-img0026.jpg)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. Novus's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary.](http://images.novusbio.com/fullsize2/IkB-alpha-Antibody-(6A920)-Flow-Cytometry-NB100-56507-img0025.jpg)
![Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.](http://images.novusbio.com/fullsize2/IkB-alpha-Antibody-(6A920)-Western-Blot-NB100-56507-img0027.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NB100-56724 (lanes 1 & 2) or NB100-56507 (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa.](http://images.novusbio.com/fullsize2/IkB-alpha-Antibody-(6A920)-Western-Blot-NB100-56507-img0028.jpg)
![Simple Western: IkB-alpha Antibody (6A920) [NB100-56507] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/IkB-alpha-Antibody-(6A920)-Simple-Western-NB100-56507-img0024.jpg)