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Wnt Receptor Signaling Pathway Bioinformatics

The Wnt receptor signaling pathway is a highly preserved pathway that can lead to one of three functions depending on which form of pathway is signaled by the Wnt molecule. The canonical pathway leads to regulation of gene transcription relating to development, cell polarity, and neuron formation, the non-canonical planar cell polarity pathway maintains the cytoskeleton and therefore the shape of the cell, and the calcium pathway works to keep cellular calcium levels consistent. The pathway begins with a Wnt signaling molecule, which causes the Dsh molecule to bond on the receptor FZD on the inside of the cell and become phosphorylated. The GSK-3beta complex binds to the activated Dsh, and the beta-catenin molecule is freed. When the levels of beta-catenin get high enough, they translocate to the nucleus and bind to DNA to initiate the transcription of the previously mentioned functions. The Wnt signaling pathway has been shown to play a role in embryonic development through stem cell pluripotency, and abnormal rates of this process can result in disorders including tumor progression and type II diabetes.

Top Research Reagents

We have 939 products for the study of the Wnt Receptor Signaling Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB100-619
Western Blot: RECQ1 Antibody [NB100-619] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RecQ1 antibody NB100-619 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 1 second.Immunohistochemistry: RECQ1 Antibody [NB100-619] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RecQ1 at 1:5000 (0.2 ug/mL). Detection: DAB

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

     3 Reviews

2 Publications
NB100-509
Western Blot: INTS1 Antibody [NB100-509] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT1 antibodyA300-361A used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: INTS1 Antibody [NB100-509] - Detection of human INTS1 (INT1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT1 antibody NB100-509 used for IP at 3 ug per reaction. INT1 was also immunoprecipitated by rabbit anti-INT1 antibody from Company B. For blotting immunoprecipitated INT1, NB100-509 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IP

H00004999-M01
Western Blot: Orc2 Antibody (3E11-1G5) [H00004999-M01] - ORC2L monoclonal antibody (M01), clone 3E11-1G5 Analysis of ORC2L expression in Jurkat.Immunohistochemistry-Paraffin: Orc2 Antibody (3E11-1G5) [H00004999-M01] - Analysis of monoclonal antibody to ORC2L on formalin-fixed paraffin-embedded human spleen tissue.Antibody concentration 5 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, IHC

1 Publication
NBP1-91821
Western Blot: DCI Antibody [NBP1-91821] - Analysis using Anti-ECI1 antibody NBP1-91821 (A) shows similar pattern to independent antibody NBP1-91822 (B).Immunohistochemistry-Paraffin: DCI Antibody [NBP1-91821] - Staining of human testis.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NB100-598
Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (RAY) [NB100-598] - BRCA (RAY) antibody was tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

5 Publications
NB100-353
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

8 Publications
NBP2-33609
Western Blot: SH3BGRL Antibody [NBP2-33609] - Analysis in human cell lines SK-MEL-30 and A-431 using anti-SH3BGRL antibody. Corresponding SH3BGRL RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.Immunocytochemistry/Immunofluorescence: SH3BGRL Antibody [NBP2-33609] - Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

MAB2890
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN‑ alpha A (Catalog # <a class=Daudi  human Burkitt's lymphoma cell line untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN‑ alpha A (Catalog # <a class=

Rabbit Monoclonal
Species Human
Applications WB, Simple Western, Flow

AF1120
Frizzled‑1 was detected in immersion fixed frozen sections of embryonic mouse intestine (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1120) overnight at <BR>4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Mouse
Applications WB, IHC

3 Publications
AF2390
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin‑4 at approximately 158 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Simple Western

8 Publications
AF1408
Western blot shows lysates of human colon tissue, PC‑12 rat adrenal pheochromocytoma cell line, A549 human lung carcinoma cell line, and SK‑BR‑3 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cystatin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1408) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cystatin B at approximately 18 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Rat Cystatin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1408) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Rat
Applications WB, Simple Western, IHC

2 Publications
AF2444

Goat Polyclonal
Species Human
Applications WB, IHC

     2 Reviews

5 Publications
AF6646
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human LRP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6646) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

Sheep Polyclonal
Species Human
Applications WB

AF1286
    Western  blot shows lysates of human skin tissue. PVDF membrane was probed with  1 µg/mL of Goat Anti-Human Cystatin E/M Antigen  Affinity-purified Polyclonal Antibody (Catalog # AF1286) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    Simple  Western lane view shows lysates of human skin tissue, loaded at  0.2 mg/mL. A specific band was detected for Cystatin E/M at  approximately 13 kDa (as indicated) using 50 µg/mL of Goat  Anti-Human Cystatin E/M Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF1286) followed by 1:50 dilution of HRP-conjugated  Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

     1 Review

4 Publications
AF1617

Goat Polyclonal
Species Human
Applications WB

8 Publications
NB300-284
Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0 ug; lane 2: 50 ug; lanes 3 , 7 and 9: 250 ug; lane 5: 500 ug. (anti-cMHC was used at 1:500; secondary antibody: Goat anti-mouse IgG Fc-HRP; 1:5000)Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal antibody at low microscopical magnification.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     2 Reviews

11 Publications
NBP2-38161
Immunohistochemistry-Paraffin: SH3BGR Antibody [NBP2-38161] - Staining of human skin shows low expression as expected.Immunohistochemistry-Paraffin: SH3BGR Antibody [NBP2-38161] - Staining of human heart muscle shows high expression.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

3014-NR


Species Human
Applications BA

7 Publications
NBP3-09392
Western Blot: Opsin 1 (Long Wave) Antibody [NBP3-09392] - Western blot analysis of Opsin 1 (Long Wave) in Fetal Heart lysates. Antibody dilution at 1.0ug/ml

Rabbit Polyclonal
Species Human, Mouse
Applications WB