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T Cell Differentiation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the T Cell Differentiation Pathway and Autoimmune Reaction, Autoimmune Diseases, Neoplasms, Inflammation, Autoimmunity. The study of the T Cell Differentiation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The T Cell Differentiation Pathway has been researched in relation to Cell Differentiation, Cell Development, Cell Activation, Immune Response, T Cell Activation. The T Cell Differentiation Pathway complements our catalog of research reagents including antibodies and ELISA kits against CD3, CD4, CD8A, CD28, CD80.

T Cell Differentiation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on T Cell Differentiation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 3616 products for the study of the T Cell Differentiation Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

     6 Reviews

13 Publications
202-IL/CF
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED<SUB>50</SUB> for this effect is 0.05-0.25 ng/mL.


Species Human

207 Publications
NBP1-49045
Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/ml and incubated for 3 hours at room temperature.Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.

Rat Monoclonal
Species Mouse, Rat
Applications Flow, ICC/IF, IHC

     2 Reviews

9 Publications
204-IL/CF
Recombinant Human IL-4 (Catalog # 204‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED<SUB>50</SUB> for this effect is 0.05‑02 ng/mL. 1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.


Species Human

259 Publications
285-IF/CF
1 μg/lane of Recombinant Human IFN-gamma  was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.Recombinant Human IFN-gamma  (Catalog # 285‑IF/CF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED<SUB>50</SUB> for this effect is 0.15‑0.75 ng/mL.


Species Human

219 Publications
AF-386-PB
    Western  blot shows lysates of NS0 mouse myeloma cell line either mock transfected or  transfected with human <br>CTLA-4. PVDF membrane was probed with  0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen  Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

     2 Reviews

2 Publications
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

13 Publications
H00003117-M01
Proximity Ligation Assay: HLA DQA1 Antibody (1A3) [H00003117-M01] - Analysis of protein-protein interactions between CD74 and HLA-DQA1. HeLa cells were stained with anti-CD74 rabbit purified polyclonal 1:1200 and anti-HLA-DQA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).ELISA: HLA DQA1 Antibody (1A3) [H00003117-M01] - Detection limit for recombinant GST tagged HLA-DQA1 is 0.3 ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ELISA, PLA

210-TA/CF
Recombinant Human TNF-alpha  (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human

428 Publications
NBP1-43299
Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

1 Publication
NB100-39002
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     1 Review

6 Publications
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, AgAct

24 Publications
207-IL/CF


Species Human

84 Publications
NBP2-02905
Western Blot: B7-1/CD80 Antibody (2E5) [NBP2-02905] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80.

Mouse Monoclonal
Species Human, Monkey
Applications WB, ICC/IF, IHC

     1 Review

NBP1-92684
Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Western blot of crude rat brain extract from a postnatal 3 day animal stained with NBP1-92684. Two bands at ~45kDa and ~35kDa show that NBP1-92684 binds to an epitope in the region of Doublecortin shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF