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T Cell Costimulation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the T Cell Costimulation Pathway and Autoimmune Reaction, Neoplasms, Autoimmune Diseases, Tissue Adhesions, Autoimmunity. The study of the T Cell Costimulation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The T Cell Costimulation Pathway has been researched in relation to Cell Activation, T Cell Activation, Cell Proliferation, T Cell Proliferation, Immune Response. The T Cell Costimulation Pathway complements our catalog of research reagents including antibodies and ELISA kits against CD3, APC, CD4, CD8A, CD28.

T Cell Costimulation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on T Cell Costimulation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 3470 products for the study of the T Cell Costimulation Pathway that can be applied to Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

AF-386-PB
CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=    Western  blot shows lysates of NS0 mouse myeloma cell line either mock transfected or  transfected with human <br>CTLA-4. PVDF membrane was probed with  0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen  Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

     2 Reviews

2 Publications
MAB140
Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # <A class=NoLineLink href=B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications Flow, IHC, CyTOF-ready

5 Publications
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, AgAct

24 Publications
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

13 Publications
H00003117-M01
Proximity Ligation Assay: HLA DQA1 Antibody (1A3) [H00003117-M01] - Analysis of protein-protein interactions between CD74 and HLA-DQA1. HeLa cells were stained with anti-CD74 rabbit purified polyclonal 1:1200 and anti-HLA-DQA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).ELISA: HLA DQA1 Antibody (1A3) [H00003117-M01] - Detection limit for recombinant GST tagged HLA-DQA1 is 0.3 ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ELISA, PLA

202-IL/CF
Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED<SUB>50</SUB> for this effect is 0.05-0.25 ng/mL.1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.


Species Human

207 Publications
NBP2-25208
Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

210-TA/CF
Recombinant Human TNF-alpha  (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human

428 Publications
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

     6 Reviews

13 Publications
285-IF/CF
1 μg/lane of Recombinant Human IFN-gamma  was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.Recombinant Human IFN-gamma  (Catalog # 285‑IF/CF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED<SUB>50</SUB> for this effect is 0.15‑0.75 ng/mL.


Species Human

219 Publications
204-IL/CF
1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.Recombinant Human IL-4 (Catalog # 204‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED<SUB>50</SUB> for this effect is 0.05‑02 ng/mL.


Species Human

259 Publications
NBP1-49045
Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/ml and incubated for 3 hours at room temperature.Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.

Rat Monoclonal
Species Mouse, Rat
Applications Flow, ICC/IF, IHC

     2 Reviews

9 Publications
NBP2-12499
Immunohistochemistry-Paraffin: ICOS Antibody (SP98) [NBP2-12499] - Human Reactive Lymph NodeImmunohistochemistry-Paraffin: ICOS Antibody (SP98) [NBP2-12499] - Human Tonsil.

Rabbit Monoclonal
Species Human
Applications IHC, IHC-P

AF4885

Sheep Polyclonal
Species Mouse
Applications WB, IP

1 Publication
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

DDX0130P-100
Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - Human cervical tissue. a: normal cervical epithelium. b: cervical intraepithelial neoplasia. c: squamous cell carcinoma.

Mouse Monoclonal
Species Human
Applications Flow, Func, ICC/IF

8 Publications
AF3388
    Western  blot shows lysates of human T regulatory cells (Tregs) and human peripheral  blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 1  μg/mL PHA  for 5 days. PVDF membrane was probed with 0.5 µg/mL of Sheep  Anti-Human OX40/TNFRSF4 Antigen Affinity-purified Polyclonal Antibody  (Catalog # AF3388) followed by HRP-conjugated Anti-Sheep IgG Secondary  Antibody (Catalog # <a class=Human T cells were treated for 3‑5 days with 1 μg/mL PHA then stained with Sheep Anti-Human OX40/<BR>TNFRSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3388, filled histogram) or control antibody (Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human
Applications WB, Flow, IHC