Submit your image related to Pathways to be featured!

Get Social

Submit your Twitter account related to Sterol Transport to be featured!

Blogs

Submit your blog on Sterol Transport to be featured!

Events

Submit your event on Sterol Transport to be featured!

Videos

Submit your video on Sterol Transport to be featured!

Charities

Submit your charity on Sterol Transport to be featured!

Sterol Transport Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Sterol Transport Pathway and Atherosclerosis, Niemann-pick Diseases, Hypercholesterolemia, Sitosterolemia, Cardiovascular Diseases. The study of the Sterol Transport Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Sterol Transport Pathway has been researched in relation to Transport, Cholesterol Transport, Intracellular Sterol Transport, Secretion, Cholesterol Homeostasis. The Sterol Transport Pathway complements our catalog of research reagents including antibodies and ELISA kits against OSBP, ABCG5, ABCG8, APOA1, ABCB6.

Sterol Transport Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Sterol Transport below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1109 products for the study of the Sterol Transport Pathway that can be applied to Flow Cytometry, Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP2-00935
Western Blot: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.Immunocytochemistry/Immunofluorescence: OSBP1 Antibody (1F2) [NBP2-00935] Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP1.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

2 Publications
NBP2-23488
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in (1) HL7702, (2) RAJI and (3) Jurkat cell lysate.Immunohistochemistry: ABCG5 Antibody (1B5E10) [NBP2-23488] - IHC analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.

Mouse Monoclonal
Species Human
Applications ICC/IF (-), WB, ELISA

NBP1-71706
Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.Immunocytochemistry/Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin.  The cells were incubated with anti-ABCG8 Antibody (1B10A5) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution.  Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution.  Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     1 Review

8 Publications
NBP2-52979
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-APOA1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-APOA1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

NBP2-58327
Western Blot: ABCB6 Antibody [NBP2-58327] - Analysis in human cell line RT-4 and human cell line U-251 MG.Immunocytochemistry/Immunofluorescence: ABCB6 Antibody [NBP2-58327] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF

1 Publication
NBP2-04611
Western Blot: PRB1 Overexpression Lysate (Adult Normal) [NBP2-04611] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRB1.


Species Human

NB400-148
Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, EM, ICC/IF

     5 Reviews

39 Publications
NBP2-22124
Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, ELISA, Flow

4 Publications
NB400-128
Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml.  ECL: 30 minute exposure.Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Staining of human small intestine, enterocytes.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

23 Publications
NB400-105
Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal  (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.Western Blot: ABCA1 Antibody [NB400-105] - Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ELISA

     6 Reviews

333 Publications
NB400-132
Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. 1: untreated mouse peritoneal macrophages, 2: 22-(R)-hydrocholesterol treated macrophages, 3: T0901713 treated macrophages, 4: T091713 treated macrophages.Flow (Intracellular): ABCG1 Antibody [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed with  NB400-132 (blue) and a matched isotype control NB810-56910 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Goat Anti-Rabbit Dylight 550-conjugated antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, IB

     2 Reviews

132 Publications
NBP2-67656
Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NB100-615
Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000.  Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that  demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY licence.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IB

     2 Reviews

25 Publications
NBP1-78159
Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] -  Image from a customer review on porcine intestinal epithelial cells IPEC-J2.

Mouse Monoclonal
Species Human, Rat, Porcine
Applications WB, EM, ELISA

     1 Review

20 Publications
NBP1-88527
Western Blot: ARHGAP4 Antibody [NBP1-88527] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human colon.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NB400-141

Rabbit Polyclonal
Species Human, Mouse, Hamster
Applications WB, ICC/IF, IP

4 Publications
NBP1-42342

Rat Monoclonal
Species Human
Applications WB, Flow, IHC

1 Publication
NB400-104
Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% BSA. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     4 Reviews

196 Publications