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Spindle Assembly Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Spindle Assembly Pathway and Malignant Neoplasms, Neoplasms, Aneuploidy, Cell Transformation, Neoplastic, Genomic Instability. The study of the Spindle Assembly Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Spindle Assembly Pathway has been researched in relation to Mitosis, Spindle Assembly Checkpoint, Cell Cycle, Anaphase, Chromosome Segregation. The Spindle Assembly Pathway complements our catalog of research reagents including antibodies and ELISA kits against AURORA B, AURORA A, APC, BUB1, BUB1B.

Spindle Assembly Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Spindle Assembly below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1128 products for the study of the Spindle Assembly Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2 on HeLa whole cell lysate using NB100-563. MAD2 was also immunoprecipitated using NB100-564. Immunoprecipitated MAD2 was blotted using NB100-564.

Rabbit Polyclonal
Species Human
Applications WB, IHC-P, IP

     1 Review

13 Publications
AF4185
    Mxi1  was detected in immersion fixed LNCaP human prostate cancer cell line using  Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog #  AF4185) at 15 µg/mL for 3 hours at room temperature. Cells  were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG  Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications ICC

NBP1-81765
Immunohistochemistry-Paraffin: ADCY10 Antibody [NBP1-81765] - Staining of human kidney shows strong positivity in tubules.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

AF4885

Sheep Polyclonal
Species Mouse
Applications WB, IP

1 Publication
NB100-59828
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry: Cdc20 Antibody [NB100-59828] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC-P, IP

3 Publications
NB100-353
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

6 Publications
NB100-547
Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Asynchronous HeLa cells.  Antibody used at 0.5 ug/ml (left panel) and 0.2 ug/ml (right panel). Cells were counterstained with DAPI.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IP

NBP1-32775
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ICC/IF

2 Publications
NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

3 Publications
NB500-181
Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF11.Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde.  Cytoplasmic staining during interphase and spindle staining during mitosis.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IP

     2 Reviews

12 Publications
NBP1-89979
Immunocytochemistry/Immunofluorescence: Mad Antibody [NBP1-89979] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli and mitochondria.Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

H00000699-D01P
Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, PLA

NBP1-31776
Western Blot: Ran Antibody [NBP1-31776] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody diluted at 1:1000.Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-31776] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF

     1 Review

AF6028
Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6028) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <A class=NoLineLink href=TTK was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6028) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human
Applications WB, IHC

MAB60001
    Cyclin B1  was detected in immersion fixed paraffin-embedded sections of human squamous  cell carcinoma using Rabbit Anti-Human Cyclin B1 Monoclonal Antibody  (Catalog # MAB60001) at 3 µg/mL for 1 hour at room  temperature followed by incubation with the Anti-Rabbit IgG  VisUCyte™ HRP Polymer Antibody (Catalog # <a class=    Western  blot shows lysates of Jurkat human acute T cell leukemia cell line, K562  human chronic myelogenous leukemia cell line, and U2OS human osteosarcoma  cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit  Anti-Human Cyclin B1 Monoclonal Antibody (Catalog # MAB60001)  followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody  (Catalog # <a class=

Rabbit Monoclonal
Species Human
Applications WB, Simple Western, IHC

     1 Review

NB500-179
Western Blot: TPX2 Antibody [NB500-179] - Analysis of TPX2 expression in 1) HeLa, 2) Ntera2, 3) K-562 and 4) Raji whole cell lysates using NB500-179.Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - analysis of TPX2 at the mitotic spindle microtubules and poles in a HeLa metaphase cell. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     1 Review

20 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     2 Reviews

22 Publications