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Response To Methotrexate Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Response To Methotrexate Pathway and Arthritis, Rheumatoid Arthritis, Neoplasms, Malignant Neoplasms, Infective Disorder. The study of the Response To Methotrexate Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Response To Methotrexate Pathway has been researched in relation to Transport, Pathogenesis, Cell Activation, T Cell Activation, Methotrexate Transport. The Response To Methotrexate Pathway complements our catalog of research reagents including antibodies and ELISA kits against TNF, ACR, DHFR, CRP, MTHFR.

Top Research Reagents

We have 3797 products for the study of the Response To Methotrexate Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

H00001719-M01
Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Folic acid treatment restores endothelial specific DHFR expression and activity in Ang II-infused apoE null mice.DHFR expression (A, n?=?8-11) and activity (B, n?=?5-7) measured from endothelial cells (ECs) and EC-denuded aortas isolated from apoE null mice after 4 weeks of infusion. Data show that DHFR expression and activity was restored in FA treated animals specifically in the endothelial cells. eNOS was stained to show that isolation/removal of ECs from the vessels was successful. *p<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0088899) licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of monoclonal antibody to DHFR on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

12 Publications
DCRP00B
N/A C-Reactive Protein/CRP [HRP]N/A C-Reactive Protein/CRP [HRP]


Species Human
Applications ELISA

139 Publications
D6050B
N/A IL-6 [HRP]N/A IL-6 [HRP]


Species Human

747 Publications
NBP3-25721
Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-59904
Western Blot: Reduced Folate Carrier/SLC19A1 Antibody [NBP1-59904] - Titration: 5.0ug/ml Positive Control: Jurkat cell lysate.Immunohistochemistry-Paraffin: Reduced Folate Carrier/SLC19A1 Antibody [NBP1-59904] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NB300-560
Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-Fr

     1 Review

10 Publications
NBP1-87404
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human duodenum and testis tissues using NBP1-87404 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human duodenum, small intestine, stomach and testis using Anti-Adenosine Deaminase/ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

2 Publications
NBP1-87741
Western Blot: Metaxin 1 Antibody [NBP1-87741] - Analysis in human cell line MCF-7.Immunocytochemistry/Immunofluorescence: Metaxin 1 Antibody [NBP1-87741] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-88296
Western Blot: ITPA Antibody [NBP1-88296] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp.  Lane 4: Human plasma (IgG/HSA depleted).  Lane 5: Human liver tissue.  Lane 6: Human tonsil tissueImmunocytochemistry/Immunofluorescence: ITPA Antibody [NBP1-88296] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-89192
Western Blot: ATIC Antibody [NBP1-89192] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATIC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.Immunocytochemistry/Immunofluorescence: ATIC Antibody [NBP1-89192] - Staining of human cell line A-431 shows positivity in plasma membrane and cytoplasm. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-14260
Analysis in human testis and liver tissues using NBP2-14260 antibody. Corresponding ACR RNA-seq data are presented for the same tissues.Staining of human testis show strong extracellular space and nuclear positivity in subset of cells in seminiferous ducts.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications ICC/IF, IHC, IHC-P

10 Publications
MAB1455
Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, Simple Western, IHC

     3 Reviews

32 Publications
NBP2-37607
Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mouse mAb against Rat Heart cell lysate.Immunohistochemistry-Paraffin: MTHFR Antibody (5D3) [NBP2-37607] -  Analysis of  intima cancer tissues (left) and prostate tissues (right) using MTHFR mouse mAb with DAB staining.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, IHC

NBP2-37737
Western Blot: Jak3 Antibody (5H2) [NBP2-37737] - Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).Immunocytochemistry/Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, Flow

2 Publications
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

791 Publications
DY417
N/A IL-10 [Biotin]


Species Mouse
Applications ELISA

284 Publications
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # <a class=CD28 was detected in immersion fixed U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications WB, AgAct, ICC

     1 Review

40 Publications
MAB140
Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # <a class=<P align=left>Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications Flow, IHC, CyTOF-ready

14 Publications
NBP2-45312
Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DRB1 Antibody (SPM288).

Mouse Monoclonal
Species Human, Monkey, Mouse (Negative)
Applications WB, Flow, ICC/IF

2 Publications