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Response To Hypoxia Pathway Bioinformatics

Hypoxia occurs when a region of the body is deprived of oxygen. Hypoxia is a very dangerous condition when the brain is affected, for neurons can begin to die off within a matter of minutes, especially in cases of complete oxygen deprivation, or anoxia. Anaerobic metabolism occurs when the cells are not receiving oxygen, which results in a buildup of lactic acid, and could lead to cell death if oxygen is not soon introduced. Chemoreceptors in the carotid body are able to detect changes in the oxygen level of cells, and cause a response of vasodilation, or the widening of blood vessels to increase blood flow and allow for greater perfusion. In contrast, the muscles and blood vessels in the lungs constrict, redirecting the flow of oxygen back into the alveoli to allow for a greater rate of gas exchange. Other methods of restoring oxygen levels include hyperventilation and adaptation, which is controlled by the hypoxia-inducible factor 1 (HIF-1). In response to hypoxia response elements, HIF-1 is able to control microRNAs, which can help the body adapt to low levels of oxygen through routes such as metabolic, apoptotic, proliferative, and angiogenic adaptations. The most easily seen response to hypoxia is hyperventilation, which increases the activity of the respiratory system, but may actually be detrimental to the body if the carbon dioxide levels get too low and cause the blood vessels to re-constrict and may also change the pH of the bloodstream.

Response To Hypoxia Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Response To Hypoxia below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1859 products for the study of the Response To Hypoxia Pathway that can be applied to Flow Cytometry, Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review.Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog # NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ELISA

     40 Reviews

851 Publications
DVE00
 VEGF [HRP] VEGF [HRP]


Species Human

     25 Reviews

512 Publications
MEP00B
 Erythropoietin/EPO [HRP] Erythropoietin/EPO [HRP]


Species Mouse

     3 Reviews

77 Publications
NBP2-88941
Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.

Mouse Monoclonal
Species Human
Applications IHC, IHC-P

NB100-124
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Western Blot analysis with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124], theoretical molecular weight 86.6 kDa. Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1A or HIF-2A expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1A-shRNA or HIF-2A-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. HIF-1B expression was used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY licence.Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and ARNT/HIF-1 beta antibody (H1beta234)[NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight for ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY licence.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, GS

     2 Reviews

96 Publications
DTM100
 TIMP-1 [HRP] TIMP-1 [HRP]


Species Human

     1 Review

81 Publications
NBP2-13967
Western Blot: EPX Antibody [NBP2-13967] - Analysis in human cell line NB4.Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

     1 Review

6 Publications
NB100-122
Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep21520) licensed under a CC-BY licence.Knockout Validated: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep21520) licensed under a CC-BY licence.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     31 Reviews

640 Publications
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human skeletal muscle.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP1-86615
Western Blot: CRAT Antibody [NBP1-86615] - Analysis using Anti-CRAT antibody NBP1-86615 (A) shows similar pattern to independent antibody NBP1-86616 (B).Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86615] - Staining of human tonsil shows low expression as expected.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NB300-605
Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, IB

     3 Reviews

53 Publications
MAB1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,<br>PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=    ERK2  was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast  adipose-like cell line using Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal  Antibody (Catalog # MAB1230) at 3 µg/mL for 3 hours at  room temperature. Cells were stained using the NorthernLights™  557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog #  <a class=

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza (PMID: 21620138).Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ChIP, Flow

     7 Reviews

35 Publications
NB100-62346
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. Western blot analysis for protein expressions of intrarenal RAS components in the two groups of mice. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical analysis of intrarenal RAS expression position in the two groups of mice. AGT immunoreactivity in the proximal tubular cells, Ang II immunoreactivity in both glomerular and tubular cells, renin immunoreactivity in juxtaglomerular apparatus cells, ACE immunoreactivity in brush border membranes of proximal tubules as well as AT1 and AT2 immunoreactivity in the proximal tubules were increased in the HF group when compared to the control group. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

12 Publications
NBP2-32001
Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32001] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32001] - Staining of human colon shows moderate cytoplasmic positivity in myocytes.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
DET100
 Endothelin-1 [HRP] Endothelin-1 [HRP]


Species Multi-Species

     6 Reviews

40 Publications
NBP2-26116
Western Blot: Fucosyltransferase 2/FUT2 Antibody [NBP2-26116] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.  Immunohistochemistry-Paraffin: Fucosyltransferase 2/FUT2 Antibody [NBP2-26116] -  Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow