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Posttranscriptional Gene Silencing Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Posttranscriptional Gene Silencing Pathway and Plant Diseases, Virus Diseases, Infective Disorder, Malignant Neoplasms, Neoplasms. The study of the Posttranscriptional Gene Silencing Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Posttranscriptional Gene Silencing Pathway has been researched in relation to Gene Silencing, Rna Interference, Methylation, Translation, Dna Methylation. The Posttranscriptional Gene Silencing Pathway complements our catalog of research reagents including antibodies and ELISA kits against EIF2C1, DICER1, EIF2C2, SCPEP1, LIN9.

Posttranscriptional Gene Silencing Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Posttranscriptional Gene Silencing below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1182 products for the study of the Posttranscriptional Gene Silencing Pathway that can be applied to Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP3-15868
Western Blot: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Analysis of extracts of various cell lines, using Ceruloplasmin antibody (NBP3-15868) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Immunofluorescence analysis of mouse liver using Ceruloplasmin Rabbit mAb (NBP3-15868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-71691
Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     1 Review

NB100-56648
Western Blot: RISC Antibody [NB100-56648] - Analysis of human RISC in 15 ugs of human heart cell lysate using NB100-56648 at 1:1000 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB

NBP1-33581
Immunocytochemistry/Immunofluorescence: Factor IX Antibody [NBP1-33581] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX protein stained by Factor IX antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581] - Mouse liver. Factor IX stained by Factor IX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications ICC/IF, IHC, IHC-P

NBA1-19349
Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in  with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025).  At passage 5, cells were harvested and analyzed for pluripotent markers by flow cytometry.  Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A).  Quadrant lines were drawn based on matched isotype controls.


Species Mouse
Applications Flow, ICC/IF, In vitro

13 Publications
NBP1-59631
Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/mlImmunohistochemistry: FBXW7/Cdc4 Antibody [NBP1-59631] - Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     1 Review

4 Publications
NBP1-80690
Immunocytochemistry/Immunofluorescence: LIN9 Antibody [NBP1-80690] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: LIN9 Antibody [NBP1-80690] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

2 Publications
NBP1-82859
Immunocytochemistry/Immunofluorescence: RPLP0 Antibody [NBP1-82859] - Staining of human cell line A-431 shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NBP1-85364
Western Blot: Cyclophilin 40 Antibody [NBP1-85364] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody [NBP1-85364] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NB100-1607
Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain. Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.

Chicken Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

9 Publications
NB100-2817
Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

4 Publications
AF4937
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human
Applications WB, IHC

AF1936

Goat Polyclonal
Species Human
Applications WB, IP

1 Publication
AF6367
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD302/CLEC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6367) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Human peripheral blood monocytes were stained with Sheep Anti-Human CD302/CLEC13A Affinity Purified Polyclonal Antibody (Catalog # AF6367, filled histogram) or control antibody (Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

NBP2-36776
Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HNRNPC antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in U2OS cell line with Anti-HNRNPC monoclonal antibody, showing distinct nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

1887-ML/CF


Species Mouse
Applications BA

177 Publications
NB200-111
Knockdown Validated: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT decreases p53mut stability. T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 i1/4M), Nutlin3A (5 i1/4M) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. <i>Oncotarget</i> (2014))  licensed under a CC-BY licence.Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT modulates p53 stability in-vivo and synergizes with ionizing radiation to inhibit tumor growth( Sections of PSN1 xenografts treated with three consecutive doses of MK-2206 (60 mg/kg). Sections of PSN1 xenografts and in-vitro PSN1 cells fixed and stained with anti-NPM (red) and anti-p14ARF (green). Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget (2014))  licensed under a CC-BY licence.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ELISA, Flow

     2 Reviews

17 Publications
NBP2-67121
Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysateImmunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

1 Publication
NBP1-03349
Western Blot: Drosha Antibody [NBP1-03349] - Detection of Human Drosha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Immunoprecipitation: Drosha Antibody [NBP1-03349] - Detection of human Drosha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for IP at 6 ug per reaction. Drosha was also immunoprecipitated by another rabbit anti-Drosha antibody. For blotting immunoprecipitated Drosha, NBP1-03349 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     3 Reviews

6 Publications
NBP3-11884
Immunocytochemistry/Immunofluorescence: p19 INK4d Antibody (DCS100) [NBP3-11884] - Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1).

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF