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Osteoblast Differentiation Pathway Bioinformatics

Osteoblasts are the cells that are responsible for bone formation. Conversely, osteoclasts are cells that break down bone tissue. Osteoblasts come from immature progenitor cells called osteoprogenitors, which are induced by various growth factors, including bone morphogenetic proteins (BMPs), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF) and transforming growth factor beta (TGF-beta). Together, BMPs, FGF, PDGF and TGF-beta increase bone production. In a recent study, it was reported that hESC treatment with BMP4 and FGF2 enhanced osteoblast differentiation (PMID: 23206696). The Wnt/beta-Catenin Pathway may play a role in osteoblast differentiation (PMID: 17572649).

Osteoblast Differentiation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Osteoblast Differentiation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1408 products for the study of the Osteoblast Differentiation Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NBP1-77461
Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77461] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

7 Publications
MAB1419
Osteocalcin was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=Osteocalcin was detected in immersion fixed MG‑63 human osteosarcoma cell line using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human, Rat
Applications IHC, CyTOF-ready, ICC

     2 Reviews

21 Publications
355-BM/CF


Species Human, Mouse, Rat

132 Publications
NBP1-89131
Immunocytochemistry/Immunofluorescence: ATRNL1 Antibody [NBP1-89131] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.Immunohistochemistry-Paraffin: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NB110-3638
Western Blot: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, Flow

     1 Review

11 Publications
NBP1-89133
Western Blot: ASRGL1 Antibody [NBP1-89133] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: ASRGL1 Antibody [NBP1-89133] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
NBP1-89134
Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining in human skeletal muscle and cerebral cortex tissues using anti-PDLIM3 antibody. Corresponding PDLIM3 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining of human cerebral cortex shows low expression as expected.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

1 Publication
NBP2-32263
Western Blot: NAT10 Antibody [NBP2-32263] - Samples:  Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.  Antibodies:  Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml.  Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Rabbit Polyclonal
Species Human
Applications WB, IHC, IP

AF808
Recombinant Mouse Osteopontin/OPN (Catalog # <a class=  Osteopontin/OPN  was detected in immersion fixed C2C12 mouse myoblast cell line (left panel,  positive stain) and mouse splenocytes (right panel, negative stain) using  Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody  (Catalog # AF808) at 5 µg/mL for 3 hours at room  temperature. Cells were stained using the NorthernLights™  557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog #  <a class=

Goat Polyclonal
Species Mouse
Applications WB, IHC, ELISA(Cap)

     4 Reviews

63 Publications
MAB7547
Western blot shows lysates of Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Osterix/Sp7 was detected in immersion fixed Saos‑2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, ICC

4014-SP


Species Human

2 Publications
NBP2-35212
SDS-Page: Human PTH Protein [NBP2-35212]


Species Human

6449-TEC/CF


Species Human

1 Publication
314-BP
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=Recombinant Human BMP-4 (Catalog #<BR>314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED<SUB>50</SUB> for this effect is 2.5-15 ng/mL.


Species Human

     3 Reviews

300 Publications