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Osteoblast Differentiation Pathway Bioinformatics

Osteoblasts are the cells that are responsible for bone formation. Conversely, osteoclasts are cells that break down bone tissue. Osteoblasts come from immature progenitor cells called osteoprogenitors, which are induced by various growth factors, including bone morphogenetic proteins (BMPs), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF) and transforming growth factor beta (TGF-beta). Together, BMPs, FGF, PDGF and TGF-beta increase bone production. In a recent study, it was reported that hESC treatment with BMP4 and FGF2 enhanced osteoblast differentiation (PMID: 23206696). The Wnt/beta-Catenin Pathway may play a role in osteoblast differentiation (PMID: 17572649).

Osteoblast Differentiation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Osteoblast Differentiation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1437 products for the study of the Osteoblast Differentiation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB110-3638
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY licence.Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     1 Review

12 Publications
NBP1-89131
Immunocytochemistry/Immunofluorescence: ATRNL1 Antibody [NBP1-89131] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.Immunohistochemistry-Paraffin: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NBP1-89133
Western Blot: ASRGL1 Antibody [NBP1-89133] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: ASRGL1 Antibody [NBP1-89133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
NBP1-89134
Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining in human skeletal muscle and cerebral cortex tissues using anti-PDLIM3 antibody. Corresponding PDLIM3 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining of human cerebral cortex shows low expression as expected.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

1 Publication
NBP2-32263
Western Blot: NAT10 Antibody [NBP2-32263] - Samples:  Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.  Antibodies:  Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml.  Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IP

AF808
  Osteopontin/OPN  was detected in immersion fixed C2C12 mouse myoblast cell line (left panel,  positive stain) and mouse splenocytes (right panel, negative stain) using  Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody  (Catalog # AF808) at 5 µg/mL for 3 hours at room  temperature. Cells were stained using the NorthernLights™  557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog #  <a class=Recombinant Mouse Osteopontin/OPN (Catalog # <a class=

Goat Polyclonal
Species Mouse
Applications WB, IHC, ELISA(Cap)

     8 Reviews

99 Publications
MAB7547
Western blot shows lysates of Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Osterix/Sp7 was detected in immersion fixed Saos‑2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, ICC

     1 Review

MAB2006
RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # <A class=NoLineLink href=

Rat Monoclonal
Species Human
Applications ICC

     3 Reviews

17 Publications
MAB1419
Saos‑2 human osteosarcoma cell line was stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # <A class=NoLineLink href=Human osteoblasts were stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human, Rat
Applications IHC, CyTOF-ready, ICC

     4 Reviews

27 Publications
4014-SP


Species Human
Applications BA

3 Publications
462-TR/CF
1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.<P align=left>Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR/CF) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED<SUB>50</SUB> for this effect is 5-15 ng/mL in the presence of 2.5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # <A class=NoLineLink href=


Species Mouse
Applications BA

81 Publications
DY805
 Osteoprotegerin/TNFRSF11B [Biotin]


Species Human
Applications ELISA

72 Publications
DPI00
 SLPI [HRP] SLPI [HRP]


Species Human
Applications ELISA

30 Publications
DCC270
 CCL27/CTACK [HRP] CCL27/CTACK [HRP]


Species Human
Applications ELISA

10 Publications
355-BM/CF
Recombinant human/mouse/rat BMP-2 (<a class=NoLineLink href=


Species Human, Mouse, Rat
Applications BA

168 Publications
314-BP/CF
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=Recombinant Human BMP-4 (Catalog #<BR>314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED<SUB>50</SUB> for this effect is 2.5-15 ng/mL.


Species Human
Applications BA, BA

422 Publications
MAB7665
Western blot shows lysates of human parathyroid tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <A class=NoLineLink href=PTH was detected in immersion fixed paraffin-embedded sections of human parathyroid gland using Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, IHC