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Nuclear Pore Complex Assembly Pathway Bioinformatics

Nuclear pore complexes (NPCs) are tiny holes in the nuclear envelope that allow for material to pass into and out of the nucleus. NPC assembly must occur both at the end of mitosis as well as when the nucleus and nuclear envelope expand during metabolic cell growth. The mechanism of assembly of these nuclear pore complexes is still being debated by the scientific community, but there are currently three methods that have been presented with evidence. One method of NPC assembly is that the pore complexes being to appear after the nuclear envelope regrows following nuclear division. Protein complexes called Nup complexes bind to the chromatin, and then are inserted into the double membrane, causing the two to fuse together around the protein. The idea of a prepore also exists, where several Nup complexes come together and allow the nuclear envelope to form around them during the regrowth of the membrane. The prepore would begin in the nucleus, after previously going through active transport from the cytoplasm. The final mechanism of NPC assembly occurs when more promoters are added to an existing NPC, allowing for the further growth of the pore complexes.

Nuclear Pore Complex Assembly Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Nuclear Pore Complex Assembly below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 471 products for the study of the Nuclear Pore Complex Assembly Pathway that can be applied to Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

H00055746-M01
Western Blot: NUP133 Antibody (3E8) [H00055746-M01] - NUP133 monoclonal antibody (M01), clone 3E8 Analysis of NUP133 expression in HeLa.Immunocytochemistry/Immunofluorescence: NUP133 Antibody (3E8) [H00055746-M01] - Immunofluorescence of monoclonal antibody to NUP133 on HeLa cell . [antibody concentration 10 ug/ml]

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

4 Publications
H00022932-M02
Western Blot: POMZP3 Antibody (2E7) [H00022932-M02] - POMZP3 monoclonal antibody (M02), clone 2E7. Analysis of POMZP3 expression in PC-12.Immunohistochemistry-Paraffin: POMZP3 Antibody (2E7) [H00022932-M02] - Analysis of monoclonal antibody to POMZP3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, IHC

NBP1-76926
Western Blot: Nucleoporin 107 Antibody [NBP1-76926] - A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 ug/mL.Immunocytochemistry/Immunofluorescence: Nucleoporin 107 Antibody [NBP1-76926] - Immunofluorescence of NUP107 in A549 cells with NUP107 antibody at 10 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

1 Publication
NBP1-81546
Western Blot: NUP93 Antibody [NBP1-81546] - Analysis in human cell line RH-30 and human cell line MOLT-4.Immunohistochemistry-Paraffin: NUP93 Antibody [NBP1-81546] - Staining of human kidney shows weak to moderate positivity in nuclear membrane in cells in tubules.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

1 Publication
NBP1-81725
Western Blot: Nup153 Antibody [NBP1-81725] - Analysis in human cell line K562.Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NBP1-81725] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-82960
Western Blot: NUP155 Antibody [NBP1-82960] - Analysis in human cell line HeLa and human cell line MCF-7.Immunocytochemistry/Immunofluorescence: NUP155 Antibody [NBP1-82960] - Staining of human cell line U-2 OS shows localization to nuclear membrane. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-83213
Western Blot: AGFG2 Antibody [NBP1-83213] - Analysis in control (vector only transfected HEK293T lysate) and AGFG2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: AGFG2 Antibody [NBP1-83213] - Staining in human salivary gland and skeletal muscle tissues using anti-AGFG2 antibody. Corresponding AGFG2 RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP1-85638
Western Blot: RCC1 Antibody [NBP1-85638] - Analysis using Anti-RCC1 antibody NBP1-85638 (A) shows similar pattern to independent antibody NBP1-85637 (B).Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85638] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NBP1-87952
Immunocytochemistry/Immunofluorescence: ELYS Antibody [NBP1-87952] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.Immunohistochemistry-Paraffin: ELYS Antibody [NBP1-87952] - Staining of human pancreas shows strong positivity in nuclear membrane of exocrine glandular cells.

Rabbit Polyclonal
Species Human, Bovine
Applications ChIP, ICC/IF, IHC

4 Publications
NB400-148
Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, EM, ICC/IF

46 Publications
NBP2-23718
Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.

Rabbit Polyclonal
Species Human, Bovine, Equine
Applications IHC, IHC-P

236-EG
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human
Applications BA

     26 Reviews

581 Publications
DY4517-05
 Neutrophil Elastase/ELA2 [Biotin]


Species Mouse
Applications ELISA

     2 Reviews

9 Publications
NB120-2938
Western Blot: RanBP2 Antibody [NB120-2938] - Effect of hypoxia on the interaction between HIF1alpha and BICD1. Co-immunoprecipitation of HIF1alpha, Dynein IC, Importin alpha3, RanBP2 with IgG and BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41418-018-0241-1) licensed under a CC-BY licence.Immunohistochemistry: RanBP2 Antibody [NB120-2938] - Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using NB120-2938.    Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using NB120-2938.

Rabbit Polyclonal
Species Human, Mouse, Bovine
Applications WB, IHC, IHC-P

2 Publications
NBP2-61832
Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

1 Publication
NB100-93322
Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunocytochemistry/Immunofluorescence: Nup53 Antibody [NB100-93322] - Showing nuclear co-localization with p300 in PANC-1 cells using anti-Nup53 antibody (green). Image submitted by a verified customer review.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

2 Publications
NBP2-93742
Western Blot: NUP210 Antibody [NBP2-93742] - Analysis of extracts of various cell lines, using NUP210 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.Immunocytochemistry/Immunofluorescence: NUP210 Antibody [NBP2-93742] - Analysis of U-2 OS cells using NUP210 at dilution of 1:100. Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF


Related Genes

The Nuclear Pore Complex Assembly Pathway has been researched against:

Related Diseases

The Nuclear Pore Complex Assembly Pathway has been studied in relation to diseases such as:

Related PTMs

The Nuclear Pore Complex Assembly Pathway has been studied in relation to posttranslational modifications (PTMs) including: