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Nuclear Pore Complex Assembly Pathway Bioinformatics

Nuclear pore complexes (NPCs) are tiny holes in the nuclear envelope that allow for material to pass into and out of the nucleus. NPC assembly must occur both at the end of mitosis as well as when the nucleus and nuclear envelope expand during metabolic cell growth. The mechanism of assembly of these nuclear pore complexes is still being debated by the scientific community, but there are currently three methods that have been presented with evidence. One method of NPC assembly is that the pore complexes being to appear after the nuclear envelope regrows following nuclear division. Protein complexes called Nup complexes bind to the chromatin, and then are inserted into the double membrane, causing the two to fuse together around the protein. The idea of a prepore also exists, where several Nup complexes come together and allow the nuclear envelope to form around them during the regrowth of the membrane. The prepore would begin in the nucleus, after previously going through active transport from the cytoplasm. The final mechanism of NPC assembly occurs when more promoters are added to an existing NPC, allowing for the further growth of the pore complexes.

Nuclear Pore Complex Assembly Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Nuclear Pore Complex Assembly below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 336 products for the study of the Nuclear Pore Complex Assembly Pathway that can be applied to Western Blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NB400-148
Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Hela lysate, BHK lysate, A431 lysate tested at 1:1000. Image provided by verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, EM, ICC/IF

     4 Reviews

33 Publications
MAB9167
Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.

Mouse Monoclonal
Species Human
Applications WB, Flow, IHC

     1 Review

NBP1-83213
Western Blot: AGFG2 Antibody [NBP1-83213] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: AGFG2 Antibody [NBP1-83213] - Staining in human salivary gland and skeletal muscle tissues using anti-AGFG2 antibody. Corresponding AGFG2 RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

H00022932-M02
Western Blot: POMZP3 Antibody (2E7) [H00022932-M02] - POMZP3 monoclonal antibody (M02), clone 2E7. Analysis of POMZP3 expression in PC-12.Immunohistochemistry-Paraffin: POMZP3 Antibody (2E7) [H00022932-M02] - Analysis of monoclonal antibody to POMZP3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, IHC

NBP1-76927
Western Blot: Nucleoporin 107 Antibody [NBP1-76927] - A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 ug/mL.Immunocytochemistry/Immunofluorescence: Nucleoporin 107 Antibody [NBP1-76927] - Immunofluorescence of NUP107 in A549 cells with NUP107 antibody at 10 ug/mL.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ELISA, ICC/IF

NBP2-66340


Species Human

NBP2-61832
Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NB100-93322
Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunocytochemistry/Immunofluorescence: Nup53 Antibody [NB100-93322] - Showing nuclear co-localization with p300 in PANC-1 cells using anti-Nup53 antibody (green). Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     1 Review

1 Publication
NB120-2938
Western Blot: RanBP2 Antibody [NB120-2938] - Analysis of human retinal extracts.Immunohistochemistry: RanBP2 Antibody [NB120-2938] - Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using NB120-2938.    Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using NB120-2938.

Rabbit Polyclonal
Species Human, Mouse, Bovine
Applications WB, IHC, IHC-P

1 Publication
NBP1-82960
Western Blot: NUP155 Antibody [NBP1-82960] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: NUP155 Antibody [NBP1-82960] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-87952
Immunocytochemistry/Immunofluorescence: ELYS Antibody [NBP1-87952] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green. Immunohistochemistry-Paraffin: ELYS Antibody [NBP1-87952] - Staining of human placenta shows strong nuclear membrane and cytoplasmic positivity in trophoblastic cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NBP1-81546
Western Blot: NUP93 Antibody [NBP1-81546] - Analysis in human cell line SiHa.Immunohistochemistry-Paraffin: NUP93 Antibody [NBP1-81546] - Staining of human kidney shows weak to moderate positivity in nuclear membrane in cells in tubules.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NB100-93329
Western Blot: Nup153 Antibody [NB100-93329] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-9329. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-9330.Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NB100-93329] - Formaldehyde-fixed asynchronous HeLa cells.  Antibody: Affinity purified rabbit anti-NUP153   used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross adsorbed Antibody used at a dilution of 1:100.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IP

     2 Reviews

1 Publication
H00055746-M01
Western Blot: NUP133 Antibody (3E8) [H00055746-M01] - NUP133 monoclonal antibody (M01), clone 3E8 Analysis of NUP133 expression in HeLa.Immunocytochemistry/Immunofluorescence: NUP133 Antibody (3E8) [H00055746-M01] - Immunofluorescence of monoclonal antibody to NUP133 on HeLa cell. [antibody concentration 10 ug/ml]

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

4 Publications
NBP2-23718
Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.

Rabbit Polyclonal
Species Human, Bovine, Equine
Applications IHC-P

NBP1-85638
Western Blot: RCC1 Antibody [NBP1-85638] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85638] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
236-EG
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.


Species Human

     11 Reviews

326 Publications

Related Genes

The Nuclear Pore Complex Assembly Pathway has been researched against:

Related Diseases

The Nuclear Pore Complex Assembly Pathway has been studied in relation to diseases such as:

Related PTMs

The Nuclear Pore Complex Assembly Pathway has been studied in relation to posttranslational modifications (PTMs) including: