Submit your image related to Pathways to be featured!

Get Social

Submit your Twitter account related to Nuclear Import to be featured!

Blogs

Submit your blog on Nuclear Import to be featured!

Events

Submit your event on Nuclear Import to be featured!

Videos

Submit your video on Nuclear Import to be featured!

Charities

Submit your charity on Nuclear Import to be featured!

Nuclear Import Pathway Bioinformatics

Nuclear import, or the transfer of material from the cytoplasm into the cell, can happen in two ways. Smaller molecules can enter the nucleus through the pore complexes through the simple process of diffusion, but larger molecules require the help of a nuclear import receptor, or importin. The importins bind with the transferable material (cargo) and pass through the nuclear pore complex and into the nucleus. Once inside, Ran-GTP binds to the complex, releasing from the cargo from the importin. The importin and Ran-GTP move back into the cytoplasm, where they are separated and Ran-GTP is hydrolyzed to Ran-GDP via a GTPase activating protein. The Ran-GDP is able to move back into the nucleus where it is phosphorylated by a guanine nucleotide exchange factor and is able to start the process over again.

Nuclear Import Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Nuclear Import below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1428 products for the study of the Nuclear Import Pathway that can be applied to Chromatin Immunoprecipitation, Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NBP2-61832
Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NB100-79802
Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunohistochemistry: CRM1 Antibody [NB100-79802] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ICC/IF

4 Publications
MAB1259
Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for <A class=

Mouse Monoclonal
Species Human
Applications CyTOF-reported, ICC, ICFlow

     4 Reviews

52 Publications
NB400-148
Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Hela lysate, BHK lysate, A431 lysate tested at 1:1000. Image provided by verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, EM, ICC/IF

     3 Reviews

33 Publications
NB100-56583
Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, Flow, IHC-P

     1 Review

1 Publication
NB300-731
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, Flow

     2 Reviews

7 Publications
NBP1-82520
Western Blot: NTF2 Antibody [NBP1-82520] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: NTF2 Antibody [NBP1-82520] - Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

210-TA
Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human

     21 Reviews

518 Publications
NB100-93329
Western Blot: Nup153 Antibody [NB100-93329] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-9329. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-9330.Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NB100-93329] - Formaldehyde-fixed asynchronous HeLa cells.  Antibody: Affinity purified rabbit anti-NUP153   used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross adsorbed Antibody used at a dilution of 1:100.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IP

     2 Reviews

1 Publication
NB100-1081
Western Blot: Importin-7 Antibody [NB100-1081] - Western blot of C4-2 human prostate cancer cells 72hrs post transfection with 4 siRNAs targetting Importin 7 mRNA, along with non-target siRNA and reagent-only controls.Western Blot: Importin-7 Antibody [NB100-1081] - Staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat Polyclonal
Species Human
Applications WB, PEP-ELISA, KD

     1 Review

3 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

22 Publications
NB100-93337
Western Blot: RanBP2 Antibody [NB100-93337] - Whole cell lysate (50 ug) from NIH3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibodyused for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Western Blot: RanBP2 Antibody [NB100-93337] - Detection of Human RanBP2 on HeLa whole cell lysate using NB100-93337. RanBP2 was also  immunoprecipitated by rabbit anti-RanBP2 antibody NB100-93338.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IP

1 Publication
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, ELISA, Flow

NBP1-83213
Western Blot: AGFG2 Antibody [NBP1-83213] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: AGFG2 Antibody [NBP1-83213] - Staining in human salivary gland and skeletal muscle tissues using anti-AGFG2 antibody. Corresponding AGFG2 RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

H00003836-M01
Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - KPNA1 monoclonal antibody (M01), clone 2A4-1B5 Analysis of KPNA1 expression in HeLa.Immunohistochemistry-Paraffin: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - Analysis of monoclonal antibody to KPNA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, ICC/IF

     1 Review

6 Publications
NBP2-45731
Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Mouse Monoclonal
Species Human, Canine, Monkey
Applications WB, IHC, IHC-P