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Nodal Signaling Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Nodal Signaling Pathway and Malignant Neoplasms, Neoplasms, Bisphosphonate-associated Osteonecrosis, Neoplasm Metastasis, Melanoma. The study of the Nodal Signaling Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Nodal Signaling Pathway has been researched in relation to Endoderm Formation, Gastrulation, Mesoderm Formation, Menstruation, Cell Differentiation. The Nodal Signaling Pathway complements our catalog of research reagents including antibodies and ELISA kits against NODAL, SMAD2, LEFTY, TDGF1, FOXH1.

Nodal Signaling Pathway Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Nodal Signaling Pathway below! For more information on how to use Laverne, please read the How to Guide.
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Top Research Reagents

We have 952 products for the study of the Nodal Signaling Pathway that can be applied to Flow Cytometry, Chromatin Immunoprecipitation, Western Blot, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

3218-ND/CF


Species Human

13 Publications
AF3797
Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ChIP

     3 Reviews

12 Publications
NB100-1598
Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO  antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

3 Publications
AF4248

Sheep Polyclonal
Species Human
Applications WB, ICC

2 Publications
H00005308-M01
Immunocytochemistry/Immunofluorescence: PITX2 Antibody (2G6) [H00005308-M01] - Analysis of monoclonal antibody to PITX2 on HeLa cell. Antibody concentration 10 ug/ml.ELISA: PITX2 Antibody (2G6) [H00005308-M01] - Detection limit for recombinant GST tagged PITX2 is approximately 0.03ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ChIP, ELISA

4 Publications
MAB5771

Mouse Monoclonal
Species Rat
Applications WB, Flow, CyTOF-ready

     1 Review

236-EG
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human

     19 Reviews

454 Publications
7754-BH/CF


Species Human

5 Publications
AF222
    Western  blot shows lysates of A549 human lung carcinoma cell line, HeLa human  cervical epithelial carcinoma cell line, and HEK293T human embryonic kidney  cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human  Activin RIB/ALK‑4 Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF222) followed by HRP-conjugated Anti-Goat IgG Secondary  Antibody (Catalog # <a class=    Western  blot shows lysates of HeLa human cervical epithelial carcinoma parental cell  line and Activin RIB/ALK‑4 knockout HeLa cell line (KO). PVDF membrane was  probed with 1 µg/mL of Goat Anti-Human  Activin RIB/ALK‑4 Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF222) followed by HRP-conjugated Anti-Goat IgG Secondary  Antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Block, KO

3 Publications
NBP1-81002
Western Blot: Nicalin Antibody [NBP1-81002] - Analysis of mouse brain lysate. Image from verified customer review.Immunohistochemistry-Paraffin: Nicalin Antibody [NBP1-81002] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Rabbit Polyclonal
Species Human, Mouse
Applications IHC, IHC-P

     1 Review

1 Publication
AF1924
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Simple Western, ChIP

     7 Reviews

159 Publications
NB100-56479
Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a  band around 55 Kda. 1:1000 dilution, 1 hour blocking in milk 5%. Image from a verified customer review.Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Smad3 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

2 Publications
NB100-2379
Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY licence.Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     2 Reviews

17 Publications
NBP2-59320
Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review. Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16.  Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     2 Reviews

1 Publication
MAB994

Rat Monoclonal
Species Mouse
Applications WB

1 Publication
NBP2-66969
Western Blot: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of NDRG1 on SW480 cell (1) and mouse eye tissue (2) lysate using anti-NDRG1 antibody at 1/1,000 dilution.Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (JM32-02) [NBP2-66969] - Staining NDRG1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
314-BP
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=Recombinant Human BMP-4 (Catalog #<BR>314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED<SUB>50</SUB> for this effect is 2.5-15 ng/mL.


Species Human

     8 Reviews

358 Publications
NBP2-74403
Western Blot: STK38L Antibody (OTI4D8) - Azide and BSA Free [NBP2-74403] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK38L.Immunocytochemistry/Immunofluorescence: STK38L Antibody (OTI4D8) - Azide and BSA Free [NBP2-74403] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY STK38L.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC