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Myoblast Differentiation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Myoblast Differentiation Pathway and Tissue Adhesions, Dystrophy, Muscular Dystrophy, Hypertrophy, Myopathy. The study of the Myoblast Differentiation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Myoblast Differentiation Pathway has been researched in relation to Cell Differentiation, Regeneration, Cell Cycle, Myoblast Fusion, Myoblast Proliferation. The Myoblast Differentiation Pathway complements our catalog of research reagents including antibodies and ELISA kits against AKT1, C2, CDKN1A, DMPK, FGF2.

Myoblast Differentiation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Myoblast Differentiation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1610 products for the study of the Myoblast Differentiation Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NB100-56510
Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line (Rh30). Negative cell lines were PFSK-1, EB2, SK-N-SH, and SJSA-1.Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Staining of Myogenin in formalin-fixed, paraffin-embedded human myocytes using NB100-56703  at 2.5 ug/ml.  Hematoxylin-eosin counterstain.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

5 Publications
NB100-56511
Western Blot: MyoD Antibody (5.8A) [NB100-56511] -  Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MyoD1 (5.8A) NB100-56511 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

7 Publications
291-G1
1 μg/lane of Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED<SUB>50</SUB> for this effect is 0.3‑1.5 ng/mL.


Species Human

     1 Review

87 Publications
292-G2
1 μg/lane of Recombinant Human IGF-II was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.Recombinant Human IGF-II (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED<SUB>50</SUB> for this effect is 1.5‑6 ng/mL.


Species Human

     1 Review

26 Publications
NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-AKT1 (NBP2-01725) and 2 ug/ml pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

8 Publications
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

11 Publications
NBP2-20216
Western Blot: RPL6 Antibody [NBP2-20216] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: RPL6 Antibody [NBP2-20216] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-58707
Western Blot: MED10 Antibody [NBP2-58707] - Western blot analysis in control (vector only transfected HEK293T lysate) and MED10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: MED10 Antibody [NBP2-58707] - Staining of human cell line HeLa shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF

NBP1-76549
Western Blot: TM4SF1/L6 Antibody [NBP1-76549] - Human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 ug/mL.Immunocytochemistry/Immunofluorescence: TM4SF1/L6 Antibody [NBP1-76549] - Immunofluorescence of TM4SF1 in human lung tissue with TM4SF1 antibody at 20 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

NBP2-44245
Western Blot: MYF-5 Antibody (OTI2G5) [NBP2-44245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYF5, (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYF5Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Human tonsil within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Mouse Monoclonal
Species Human
Applications WB, IHC, IHC-P

AF1936

Goat Polyclonal
Species Human
Applications WB, IP

NBP2-36776
Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Lane 1: Marker  [kDa].  Lane 2:Human cell line U-251 MGImmunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in U251 cell line showing distinct nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NBP2-34260
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Mouse Monoclonal
Species Human, Mouse, Porcine
Applications WB, Flow, ICC/IF

     4 Reviews

788-G8/CF
1 μg/lane of Recombinant Human/Mouse/Rat GDF-8/Myostatin was resolved with<br>SDS-PAGE under reducing (R) and<br>non-reducing (NR) conditions and visualized by silver staining, showing the main bands at 12 kDa and 24 kDa, respectively.Recombinant Human/Mouse/Rat GDF-8/<BR>Myostatin (Catalog # 788-G8/CF) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED<SUB>50</SUB> for this effect is 2-10 ng/mL.


Species Human, Mouse, Rat

21 Publications
H00004205-M02
Western Blot: MEF2A Antibody (1A10) [H00004205-M02] - MEF2A monoclonal antibody (M02), clone 1A10. Analysis of MEF2A expression in human lung cancer.Immunocytochemistry/Immunofluorescence: MEF2A Antibody (1A10) [H00004205-M02] - Analysis of monoclonal antibody to MEF2A on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

MAB6967
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications WB

233-FB/CF
1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.Recombinant Human FGF basic (146aa) (Catalog # 233-FB/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED<sub>50</sub> for this effect is 0.1-0.6 ng/mL.


Species Human

179 Publications
MAB4470
Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Multi-Species
Applications WB, ICC

     2 Reviews

16 Publications