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Metaphase Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Metaphase Pathway and Cytogenetic Abnormality, Neoplasms, Malignant Neoplasms, Aneuploidy, Leukemia. The study of the Metaphase Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Metaphase Pathway has been researched in relation to Mitosis, Interphase, Anaphase, Cell Cycle, Meiosis. The Metaphase Pathway complements our catalog of research reagents including antibodies and ELISA kits against CDK1, BRD2, MITF, MSLN, APC.

Metaphase Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Metaphase below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1193 products for the study of the Metaphase Pathway that can be applied to Chromatin Immunoprecipitation, Western Blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

3 Publications
NBP1-30475
Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Affinity purified rabbit anti-BRD2 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

1 Publication
NB100-56561
Western Blot: MITF Antibody (21D1418) [NB100-56561] - Analysis of A375 lysate using MITF antibody at 4 ug/ml.Western Blot: MITF Antibody (21D1418) [NB100-56561] - Analysis of HeLa lysate (35ug per lane, RIPA buffer) using MiTF antibody (NB100-56561) at 0.1ug/ml. Band detected at ~60kDa. (Expected MW of 58.2kDa according to NP_937802.1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF

1 Publication
NB110-85538
Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Detection of mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1 g/ml followed by reaction with ALP goat anti-mouse IgG and BCI:5840-6.Immunohistochemistry-Paraffin: Mesothelin Antibody (MB-G10) [NB110-85538] - Human tissue sections treated by antigen retrieval methods. Primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin.Cancer Res. 11(16):5840-6.

Mouse Monoclonal
Species Human
Applications WB, ELISA, IHC

2 Publications
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

MAB6000
Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.Immunohistochemistry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Cyclin B1 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.

Rabbit Monoclonal
Species Human
Applications ICC/IF (-), WB, Flow

1 Publication
NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2 on HeLa whole cell lysate using NB100-563. MAD2 was also immunoprecipitated using NB100-564. Immunoprecipitated MAD2 was blotted using NB100-564.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

     1 Review

13 Publications
NBP2-66987
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human liver tissue using anti-Protein C antibody. Counter stained with hematoxylin.

Rabbit Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-33466
Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp.  Lane 4: Human plasma (IgG/HSA depleted).  Lane 5: Human liver tissue.  Lane 6: Human tonsil tissueImmunocytochemistry/Immunofluorescence: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

236-EG
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.


Species Human

     12 Reviews

363 Publications
NBP2-38770
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis using Anti-PLOD1 antibody NBP2-38770 (A) shows similar pattern to independent antibody NBP2-31885 (B).Immunohistochemistry-Paraffin: PLOD1 Antibody [NBP2-38770] - Staining of human placenta shows cytoplasmic positivity in decidual cells.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

2 Publications
NBP1-86241
Western Blot: MOS Antibody [NBP1-86241] - Analysis in control (vector only transfected HEK293T lysate) and MOS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: MOS Antibody [NBP1-86241] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP1-88626
Western Blot: Mxi1 Antibody [NBP1-88626] - Analysis in control (vector only transfected HEK293T lysate) and MXI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: Mxi1 Antibody [NBP1-88626] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NB100-74502
Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NB500-106
Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     4 Reviews

27 Publications
AF5414
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Simple Western

     1 Review

1 Publication