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Metaphase Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Metaphase Pathway and Cytogenetic Abnormality, Neoplasms, Malignant Neoplasms, Aneuploidy, Leukemia. The study of the Metaphase Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Metaphase Pathway has been researched in relation to Mitosis, Interphase, Anaphase, Cell Cycle, Meiosis. The Metaphase Pathway complements our catalog of research reagents including antibodies and ELISA kits against HISTONE H1, MAPK, HISTONE H3, ABL1, APC.

Metaphase Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Metaphase below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 931 products for the study of the Metaphase Pathway that can be applied to Western Blot, Flow Cytometry, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

3 Publications
NBP1-30475
Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Affinity purified rabbit anti-BRD2 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

1 Publication
NB100-56561
Western Blot: MITF Antibody (21D1418) [NB100-56561] - Analysis of A375 lysate using MITF antibody at 4 ug/ml.Western Blot: MITF Antibody (21D1418) [NB100-56561] - Western blot analysis of HeLa lysate (35ug per lane, RIPA buffer) using MiTF antibody (NB100-56561) at 0.1ug/ml. Band detected at ~60kDa. (Expected MW of 58.2kDa according to NP_937802.1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB

1 Publication
NB110-85538
Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Detection of mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1 g/ml followed by reaction with ALP goat anti-mouse IgG and BCI:5840-6.Immunohistochemistry-Paraffin: Mesothelin Antibody (MB-G10) [NB110-85538] - Human tissue sections treated by antigen retrieval methods. Primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin.Cancer Res. 11(16):5840-6.

Mouse Monoclonal
Species Human
Applications WB, ELISA, IHC

2 Publications
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

MAB60001
    Cyclin B1  was detected in immersion fixed paraffin-embedded sections of human squamous  cell carcinoma using Rabbit Anti-Human Cyclin B1 Monoclonal Antibody  (Catalog # MAB60001) at 3 µg/mL for 1 hour at room  temperature followed by incubation with the Anti-Rabbit IgG  VisUCyte™ HRP Polymer Antibody (Catalog # <a class=    Western  blot shows lysates of Jurkat human acute T cell leukemia cell line, K562  human chronic myelogenous leukemia cell line, and U2OS human osteosarcoma  cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit  Anti-Human Cyclin B1 Monoclonal Antibody (Catalog # MAB60001)  followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody  (Catalog # <a class=

Rabbit Monoclonal
Species Human
Applications WB, Simple Western, IHC

     1 Review

NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2 on HeLa whole cell lysate using NB100-563. MAD2 was also immunoprecipitated using NB100-564. Immunoprecipitated MAD2 was blotted using NB100-564.

Rabbit Polyclonal
Species Human
Applications WB, IHC-P, IP

     1 Review

13 Publications
AF4885

Sheep Polyclonal
Species Mouse
Applications WB, IP

1 Publication
NBP1-31541
Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP1-31541] - Rat2 whole cell lysate/extract.Immunocytochemistry: DNA Polymerase delta, catalytic subunit Antibody [NBP1-31541] - Immunofluorescence:  [NBP1-31541] - Analysis of HeLa, using NBP1-31541 at 1:200 dilution.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ICC/IF, IHC

236-EG
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.


Species Human

     8 Reviews

256 Publications
NBP2-38770
Western Blot: PLOD1 Antibody [NBP2-38770] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MGImmunohistochemistry-Paraffin: PLOD1 Antibody [NBP2-38770] - Staining of human placenta shows cytoplasmic positivity in decidual cells.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
NBP1-86241
Western Blot: MOS Antibody [NBP1-86241] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: MOS Antibody [NBP1-86241] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

AF4185
    Mxi1  was detected in immersion fixed LNCaP human prostate cancer cell line using  Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog #  AF4185) at 15 µg/mL for 3 hours at room temperature. Cells  were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG  Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications ICC

NB100-547
Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Asynchronous HeLa cells.  Antibody used at 0.5 ug/ml (left panel) and 0.2 ug/ml (right panel). Cells were counterstained with DAPI.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IP

NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NB500-106
Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     4 Reviews

22 Publications
AF5414
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Simple Western

1 Publication