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Mapk Cascade Pathway Bioinformatics

The MAPK pathway is made up of multiple cascades which can lead to a variety of cellular functions including proliferation, differentiation, apoptosis, and cell survival. There are a number of different stimuli and factors that can initiate the MAPK cascades, such as growth factors that bind to RTKs, proinflammatory cytokines, heat shock, and osmotic stress. The signaling molecule causes dimerization of the RTKs, which causes the binding of GRB2 and SOS leading to the Ras-GDP complex to become Ras-GTP. This initiates a phosphorylation cascade of molecules such as MEK and ERK, which activate transcription factors in the AP1 family and begin the process of growth and proliferation. In typical instances of the MAPK pathway, the Ras-GTP complex is inactivated shortly after its activation. When this process is deregulated, it can lead to the increase in the proliferation and cell survival that is seen in progressing tumors.

Mapk Cascade Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Mapk Cascade below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 763 products for the study of the Mapk Cascade Pathway that can be applied to Chromatin Immunoprecipitation, Western Blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NBP2-67358
Western Blot: MEK1 Antibody (SZ22-01) [NBP2-67358] - Whole cell lysates (30 ug per lane) from three different hematological cancer cell lines. Antibody at 1:1000. Western blot image submitted by a verified customer review.Immunocytochemistry/Immunofluorescence: MEK1 Antibody (SZ22-01) [NBP2-67358] - Staining MEK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     1 Review

AF467
    Simple  Western lane view shows lysates of COLO 205 human colorectal  adenocarcinoma cell line and MBA‑MB‑468 human breast  cancer cell line, loaded at 0.2 mg/mL. A specific band was detected  for EphB2 at approximately 139 and 146 kDa (as indicated) using  20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified  Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    Western  blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog #  <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     9 Reviews

32 Publications
MAB4540
Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Raf‑1 was detected in immersion fixed paraffin-embedded sections of human liver array using Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC

     2 Reviews

1 Publication
NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-19985
Western Blot: PSMC6 Antibody [NBP2-19985] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: PSMC6 Antibody [NBP2-19985] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

236-EG
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human

     19 Reviews

452 Publications
MAB17761
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Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC

     2 Reviews

3 Publications
AF1347
Western blot shows recombinant human p38 beta, p38 gamma, p38δ and p38 alpha  (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

5 Publications
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, ELISA, Flow

H00008814-M01
Western Blot: CDKL1 Antibody (5B11) [H00008814-M01] - CDKL1 monoclonal antibody (M01), clone 5B11. Analysis of CDKL1 expression in human stomach.Immunocytochemistry/Immunofluorescence: CDKL1 Antibody (5B11) [H00008814-M01] - Analysis of monoclonal antibody to CDKL1 on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

NBP1-91214
Western Blot: CDK20 Antibody [NBP1-91214] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG spImmunohistochemistry-Paraffin: CDK20 Antibody [NBP1-91214] - Staining of human Fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
NBP1-88792
Western Blot: NUP43 Antibody [NBP1-88792] - Analysis using Anti-NUP43 antibody NBP1-88792 (A) shows similar pattern to independent antibody NBP1-88793 (B).Immunohistochemistry-Paraffin: NUP43 Antibody [NBP1-88792] - Staining of human colon.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NBP1-82956
Western Blot: ZHX2 Antibody [NBP1-82956] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-651Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-82956] - Staining of human cell line REH shows localization to nucleoplasm & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

AF8691
Western blot shows recombinant human p38 beta, p38 gamma, p38δ and Recombinant Human Active p38 alpha  (Catalog # <a class=Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha  at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the<br>12-230 kDa separation system.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     2 Reviews

3 Publications