Submit your image related to Pathways to be featured!

Get Social

Submit your Twitter account related to Mapk Cascade to be featured!

Blogs

Submit your blog on Mapk Cascade to be featured!

Events

Submit your event on Mapk Cascade to be featured!

Videos

Submit your video on Mapk Cascade to be featured!

Charities

Submit your charity on Mapk Cascade to be featured!

Mapk Cascade Pathway Bioinformatics

The MAPK pathway is made up of multiple cascades which can lead to a variety of cellular functions including proliferation, differentiation, apoptosis, and cell survival. There are a number of different stimuli and factors that can initiate the MAPK cascades, such as growth factors that bind to RTKs, proinflammatory cytokines, heat shock, and osmotic stress. The signaling molecule causes dimerization of the RTKs, which causes the binding of GRB2 and SOS leading to the Ras-GDP complex to become Ras-GTP. This initiates a phosphorylation cascade of molecules such as MEK and ERK, which activate transcription factors in the AP1 family and begin the process of growth and proliferation. In typical instances of the MAPK pathway, the Ras-GTP complex is inactivated shortly after its activation. When this process is deregulated, it can lead to the increase in the proliferation and cell survival that is seen in progressing tumors.

Mapk Cascade Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Mapk Cascade below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 476 products for the study of the Mapk Cascade Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NBP1-47833
Western Blot: MEK1 Antibody (1F5) [NBP1-47833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK1.Immunocytochemistry/Immunofluorescence: MEK1 Antibody (1F5) [NBP1-47833] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK1.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

1 Publication
AF467
    COLO 205  human colorectal adenocarcinoma cell line was stained with Goat  Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog  # AF467, filled histogram) or isotype control antibody (Catalog #<br><a class=    D3  mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled  histogram) or isotype control antibody (Catalog #<br><a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     8 Reviews

26 Publications
MAB4540
Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Raf‑1 was detected in immersion fixed paraffin-embedded sections of human liver array using Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC

NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

H00005706-M02
Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - PSMC6 monoclonal antibody (M02), clone 2C4. Analysis of PSMC6 expression in NIH/3T3.Immunocytochemistry/Immunofluorescence: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of monoclonal antibody to PSMC6 on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

236-EG
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.


Species Human

     10 Reviews

304 Publications
MAB17761
<p align=<p align=

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC

     2 Reviews

3 Publications
AF1347
Western blot shows recombinant human p38 beta, p38 gamma, p38δ and p38 alpha  (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

4 Publications
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, ELISA, Flow

NBP2-32482
Western Blot: CDKL1 Antibody [NBP2-32482] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: CDKL1 Antibody [NBP2-32482] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-91214
Western Blot: CDK20 Antibody [NBP1-91214] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG spImmunocytochemistry/Immunofluorescence: CDK20 Antibody [NBP1-91214] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-88792
Western Blot: NUP43 Antibody [NBP1-88792] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Immunohistochemistry-Paraffin: NUP43 Antibody [NBP1-88792] - Staining of human cerebral cortex shows cytoplasmic and nuclear positivity in neuronal cells and glial cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NBP1-82956
Western Blot: ZHX2 Antibody [NBP1-82956] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-651Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-82956] - Staining of human cell line REH shows localization to nucleoplasm & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

AF8691
Western blot shows recombinant human p38 beta, p38 gamma, p38δ and Recombinant Human Active p38 alpha  (Catalog # <a class=Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha  at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the<br>12-230 kDa separation system.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

3 Publications