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Limb Bud Formation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Limb Bud Formation Pathway and Congenital Abnormality, Overlying Behavior, Teratogenic Effect, Polydactyly, Arthritis. The study of the Limb Bud Formation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Limb Bud Formation Pathway has been researched in relation to Limb Development, Localization, Cell Death, Myoblast Migration, Cell Migration. The Limb Bud Formation Pathway complements our catalog of research reagents including antibodies and ELISA kits against FGF10, FGF8, SHH, TNF, BMP4.

Limb Bud Formation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Limb Bud Formation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1545 products for the study of the Limb Bud Formation Pathway that can be applied to Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

345-FG/CF
1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.Recombinant Human FGF-10 (Catalog # 345-FG/CF) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED<SUB>50</SUB> for this effect is 20‑100 ng/mL.


Species Human

68 Publications
423-F8/CF
1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.Recombinant Human/Mouse FGF-8b (Catalog # 423-F8/CF) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED<sub>50</sub> for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.


Species Human, Mouse

46 Publications
464-SH/CF
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater than the top competitor's Sonic Hedgehog.1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.


Species Mouse

43 Publications
210-TA/CF
1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.Recombinant Human TNF-alpha  (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human

670 Publications
314-BP/CF
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=Recombinant Human BMP-4 (Catalog #<BR>314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED<SUB>50</SUB> for this effect is 2.5-15 ng/mL.


Species Human

390 Publications
NBP2-45731
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Mouse Monoclonal
Species Human, Canine, Monkey
Applications WB, IHC, IHC-P

5 Publications
7460-F4/CF


Species Human

2 Publications
NBP2-93167
Western Blot: ZP2 Antibody [NBP2-93167] - Analysis of extracts of various cell lines, using ZP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/Immunofluorescence: ZP2 Antibody [NBP2-93167] - Analysis of rat oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF

MAB7547
Western blot shows lysates of Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Osterix/Sp7 was detected in immersion fixed Saos‑2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, ICC

     1 Review

NBP1-87158
Western Blot: ALDH1A2 Antibody [NBP1-87158] - Analysis in human cell line K562.Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining in human endometrium and skin tissues. Corresponding ALDH1A2 RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

     1 Review

4 Publications
NBP2-85889
Western Blot: TBX4 Antibody [NBP2-85889] - WB Suggested Anti-TBX4 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateWestern Blot: TBX4 Antibody [NBP2-85889] - Host: Rabbit. Target: TBX4. Positive control (+): Human lung (LU). Negative control (-): MCF7 (N10). Antibody concentration: 1ug/ml

Rabbit Polyclonal
Species Human
Applications WB

H00009079-M02
Western Blot: LDB2 Antibody (1A3) [H00009079-M02] - LDB2 monoclonal antibody (M02), clone 1A3. Analysis of LDB2 expression in HeLa.Immunocytochemistry/Immunofluorescence: LDB2 Antibody (1A3) [H00009079-M02] - Analysis of monoclonal antibody to LDB2 on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

AF143
Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=NoLineLink href=  Simple  Western lane view shows lysates of human uterus and human heart tissue, loaded at  0.2 mg/mL. A specific band was detected for Decorin at approximately  140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human  Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143).  This experiment was conducted under reducing conditions and using  the 12-230 kDa separation system.        

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

17 Publications
DVE00
 VEGF [HRP] VEGF [HRP]


Species Human

     26 Reviews

537 Publications
NB600-1287
Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     1 Review

6 Publications
H00002263-M01
Western Blot: FGFR2 Antibody (1G3) [H00002263-M01] - Analysis of FGFR2 expression in transfected 293T cell line by FGFR2 monoclonal antibody (M01), clone 1G3.Lane 1: FGFR2 transfected lysate (Predicted MW: 92 KDa).Lane 2: Non-transfected lysate.Immunohistochemistry-Paraffin: FGFR2 Antibody (1G3) [H00002263-M01] - Analysis of monoclonal antibody to FGFR2 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 5 ug/ml.

Mouse Monoclonal
Species Human, Bovine
Applications WB, ELISA, ICC/IF

10 Publications
AF2085
        Western  blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6.  PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse  Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085)  followed by HRP-conjugated Anti-Goat IgG Secondary Antibody  (Catalog # <a class=Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human, Mouse
Applications WB, IHC, ChIP

     4 Reviews

90 Publications