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Kinetochore Localization Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Kinetochore Localization Pathway and Malignant Neoplasms, Aneuploidy, Neoplasms, Cell Transformation, Neoplastic, Misalignment. The study of the Kinetochore Localization Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Kinetochore Localization Pathway has been researched in relation to Localization, Mitosis, Anaphase, Spindle Checkpoint, Chromosome Segregation. The Kinetochore Localization Pathway complements our catalog of research reagents including antibodies and ELISA kits against MAD2L1, MXI1, BUB1, MAD1L1, NDC80.

Kinetochore Localization Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Kinetochore Localization below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 682 products for the study of the Kinetochore Localization Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

     1 Review

13 Publications
NBP1-88626
Western Blot: Mxi1 Antibody [NBP1-88626] - Analysis in control (vector only transfected HEK293T lysate) and MXI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: Mxi1 Antibody [NBP1-88626] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

H00000699-D01P
Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, PLA

1 Publication
NBP1-32775
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ICC/IF

2 Publications
NB100-338
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23]  diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.

Mouse Monoclonal
Species Human, Hamster
Applications WB, Flow, ICC/IF

     5 Reviews

43 Publications
NB100-353
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

8 Publications
NBP1-89979
Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining in human bone marrow and liver tissues using anti-MXD1 antibody. Corresponding MXD1 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

AF6028
Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6028) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <A class=NoLineLink href=TTK was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6028) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human
Applications WB, IHC

NBP2-75424
Western Blot: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.Positive control:Lane 1: A549        Lane 2: HL-60Lane 3: A431        Lane 4: Rat colonImmunocytochemistry/Immunofluorescence: Bub3 Antibody (JG38-81) [NBP2-75424] - Staining Bub3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NBP1-82875
Immunocytochemistry/Immunofluorescence: CENPE Antibody [NBP1-82875] - Staining of human cell line A-431 shows localization to cytosol & microtubules.Immunohistochemistry-Paraffin: CENPE Antibody [NBP1-82875] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NBP1-81765
Immunohistochemistry-Paraffin: ADCY10 Antibody [NBP1-81765] - Staining of human kidney shows strong positivity in tubules.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB100-74502
Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NB100-1103
Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103] -  Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC-P, PEP-ELISA

1 Publication
NB100-2364
Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.Immunocytochemistry/Immunofluorescence: ZWINT Antibody [NB100-2364] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC  conjugated used at a dilution of 1:100.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-38644
Western Blot: ZW10 Antibody [NBP2-38644] - Analysis using Anti-ZW10 antibody NBP2-38644 (A) shows similar pattern to independent antibody NBP2-38751 (B).Immunocytochemistry/Immunofluorescence: ZW10 Antibody [NBP2-38644] - Staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NB100-56479
Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a  band around 55 Kda. 1:1000 dilution, 1 hour blocking in milk 5%. Image from a verified customer review.Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Smad3 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC-P

     1 Review

2 Publications
NB100-1116

Goat Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

1 Publication
NB100-56507
Knockout Validated: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

33 Publications
NB100-59828
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] -   Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20  used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

4 Publications
NBP1-52937
Western Blot: CENPA Antibody [NBP1-52937] - Titration: 1.25ug/ml Positive Control: Jurkat cell lysate.Immunohistochemistry-Paraffin: CENPA Antibody [NBP1-52937] - Human Skin Tissue Squamous epithelial cells (Indicated with Arrows), 4-8ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P