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Kinetochore Assembly Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Kinetochore Assembly Pathway and Aneuploidy, Malignant Neoplasms, Stenosis, Neoplasms, Instabilities, Chromosomal. The study of the Kinetochore Assembly Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Kinetochore Assembly Pathway has been researched in relation to Chromosome Segregation, Mitosis, Localization, Cell Cycle, Interphase. The Kinetochore Assembly Pathway complements our catalog of research reagents including antibodies and ELISA kits against CENPA, CENPC1, NDC80, SUGT1, MIS12.

Kinetochore Assembly Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Kinetochore Assembly below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 369 products for the study of the Kinetochore Assembly Pathway that can be applied to Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP2-12892
Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF

NBP2-75438
Western Blot: CENPC Antibody (JG36-15) [NBP2-75438] - Western blot analysis of CENPC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lane 3: K562 cell lysateImmunocytochemistry/Immunofluorescence: CENPC Antibody (JG36-15) [NBP2-75438] - Staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NB100-338
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23]  diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.

Mouse Monoclonal
Species Human, Mouse, Hamster
Applications WB, Flow, ICC/IF

     5 Reviews

46 Publications
H00010910-M04
Western Blot: SUGT1 Antibody (1A10) [H00010910-M04] - Analysis of SUGT1 expression in Raw 264.7 (Cat # L024V1).Western Blot: SUGT1 Antibody (1A10) [H00010910-M04] - Analysis of SUGT1 expression in PC-12 (Cat # L012V1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IP

NB100-2359
Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Immunohistochemistry: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (NB100-2359). Detection: DAB

Rabbit Polyclonal
Species Human
Applications WB, IHC, IP

2 Publications
H00001059-B01P
Western Blot: CENPB Antibody [H00001059-B01P] - Analysis of CENPB expression in A-431.Immunocytochemistry/Immunofluorescence: CENPB Antibody [H00001059-B01P] - Analysis of purified antibody to CENPB on HeLa cell. (antibody concentration 10 ug/ml)

Mouse Polyclonal
Species Human
Applications WB, ICC/IF

2 Publications
H00006449-M06
Western Blot: SGTA Antibody (2E11) [H00006449-M06] - SGTA expression in PC-12 ( Cat # L012V1 ).Immunocytochemistry/Immunofluorescence: SGTA Antibody (2E11.) [H00006449-M06] - SGTA Antibody (2E11) [H00006449-M06] - Analysis of monoclonal antibody to SGTA on HeLa cell. Antibody concentration 10 ug/ml

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

NBP2-33278
Western Blot: GCR2 Antibody [NBP2-33278] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG spImmunocytochemistry/Immunofluorescence: GCR2 Antibody [NBP2-33278] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

H00002491-B01P
Western Blot: CENPI Antibody [H00002491-B01P] - Analysis of CENPI expression in transfected 293T cell line by CENPI polyclonal antibody.  Lane 1: FSHPRH1 transfected lysate(57.42 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/Immunofluorescence: CENPI Antibody [H00002491-B01P] - Analysis of purified antibody to FSHPRH1 on HeLa cell. (antibody concentration 10 ug/ml)

Mouse Polyclonal
Species Human
Applications WB, ICC/IF

NBP1-82546
Western Blot: CENPH Antibody [NBP1-82546] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-119Immunocytochemistry/Immunofluorescence: CENPH Antibody [NBP1-82546] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

14 Publications
NBP1-82875
Immunocytochemistry/Immunofluorescence: CENPE Antibody [NBP1-82875] - Staining of human cell line A-431 shows localization to cytosol & microtubules.Immunohistochemistry-Paraffin: CENPE Antibody [NBP1-82875] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NB500-101
Knockdown Validated: CENPF Antibody [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch.  The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1.  The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem  (2018)Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.

Rabbit Polyclonal
Species Human, Mouse, Bovine
Applications WB, Flow, ICC/IF

     2 Reviews

51 Publications
NB100-353
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

8 Publications
NBP3-07374
Immunocytochemistry/Immunofluorescence: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374] - Immunofluorescence analysis of PFA-fixed HeLa cells. Mxi1 Mouse Monoclonal Antibody (PCRP-Mxi1-1A3)Flow Cytometry: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374] - Flow Cytometric Analysis of PFA-fixed HeLa cells. Mxi1 Mouse Monoclonal Antibody (PCRP-Mxi1-1A3) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

NBP2-46617
Western Blot: CNPase Antibody (CL2887) [NBP2-46617] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10.  Lane 2: Mouse Cerebral Cortex tissueImmunocytochemistry/Immunofluorescence: CNPase Antibody (CL2887) [NBP2-46617] - Staining of RH-30 cells using the Anti-CNP monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC