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Intraflagellar Transport Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Intraflagellar Transport Pathway and Bardet-biedl Syndrome, Kidney Diseases, Polycystic Kidney Diseases, Retinal Degeneration, Cystic Kidney Diseases. The study of the Intraflagellar Transport Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Intraflagellar Transport Pathway has been researched in relation to Transport, Localization, Cell Cycle, Cell Division, Protein Transport. The Intraflagellar Transport Pathway complements our catalog of research reagents including antibodies and ELISA kits against IFT88, SHH, KIF3A, KIF2A, TG737RPW.

Top Research Reagents

We have 541 products for the study of the Intraflagellar Transport Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

H00003077-M01
Western Blot: HFE Antibody (1G12) [H00003077-M01] - Analysis of HFE expression in transfected 293T cell line by HFE monoclonal antibody (M01), clone 1G12.Lane 1: HFE transfected lysate(40.1 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/Immunofluorescence: HFE Antibody (1G12) [H00003077-M01] - Analysis of monoclonal antibody to HFE on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

     2 Reviews

1 Publication
NB100-2475
Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.Immunocytochemistry/Immunofluorescence: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Goat Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

4 Publications
NBP1-90686
Immunohistochemistry-Paraffin: IFT172 Antibody [NBP1-90686] Staining of human lateral ventricle shows strong cytoplasmic positivity in astrocytes.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NBP2-02313
Western Blot: HIPPI Antibody (4F6) [NBP2-02313] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIPPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIPPI.Immunocytochemistry/Immunofluorescence: HIPPI Antibody (4F6) [NBP2-02313] Staining of COS7 cells transiently transfected by pCMV6-ENTRY HIPPI.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

AF3690
Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI‑3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human, Mouse
Applications WB, ChIP, ICC

     3 Reviews

45 Publications
464-SH/CF
Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus (Catalog # 464-SH/CF) has a molecular weight (MW) of 20.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater than the top competitor's Sonic Hedgehog.


Species Mouse
Applications BA

62 Publications
H00057728-B01P
Western Blot: WDR19 Antibody [H00057728-B01P] - Analysis of WDR19 expression in transfected 293T cell line by WDR19 polyclonal antibody.  Lane 1: WDR19 transfected lysate(48.07 KDa). Lane 2: Non-transfected lysate.

Mouse Polyclonal
Species Human, Mouse
Applications WB, ICC/IF

4 Publications
NBP2-55756
Immunohistochemistry-Paraffin: IFT52 Antibody [NBP2-55756] - Staining in human fallopian tube and liver tissues using anti-IFT52 antibody. Corresponding IFT52 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: IFT52 Antibody [NBP2-55756] - Staining of human liver shows low expression as expected.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB600-600
Knockdown Validated: GLI-1 Antibody - BSA Free [NB600-600] - GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and LYVE1 in tumour sections from mice in the two groups. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.

Rabbit Polyclonal
Species Human, Mouse, Canine
Applications WB, ELISA, IB

     1 Review

50 Publications
MAB10276
    Smoothened  was detected in immersion fixed paraffin-embedded sections of human liver  cancer tissue using Mouse Anti-Human Smoothened Monoclonal Antibody (Catalog  # MAB10276) at 1.7 µg/mL for 1 hour at room temperature  followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP  Polymer Antibody (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications IHC

NBP2-98642
Western Blot: IFT20 Antibody [NBP2-98642] - Anti-IFT20 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 17 kDaImmunohistochemistry-Paraffin: IFT20 Antibody [NBP2-98642] - Staining of human IFT20 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

H00000583-P01
SDS-Page: Recombinant Human BBS2 Protein [H00000583-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA

NBP3-04509
Western Blot: SMOX Antibody [NBP3-04509] - Western blot analysis of extracts of various cell lines, using SMOX antibody (NBP3-04509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunohistochemistry-Paraffin: SMOX Antibody [NBP3-04509] - Immunohistochemistry of paraffin-embedded rat brain using SMOX Rabbit pAb (NBP3-04509) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

1 Publication
NBP3-04986
Western Blot: KIF17 Antibody [NBP3-04986] - Analysis of extracts of various cell lines, using KIF17 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitImmunohistochemistry-Paraffin: KIF17 Antibody [NBP3-04986] - Immunohistochemistry of paraffin-embedded Rat testis using KIF17 Rabbit pAb (NBP3-04986) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP3-05030
Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitImmunocytochemistry/Immunofluorescence: KIF3A Antibody [NBP3-05030] - Analysis of MCF7 cells using KIF3A antibody . Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NB500-180
Knockout Validated: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:10000 of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NB500-180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

18 Publications
H00002523-P01
12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA