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Hyperphosphorylation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Hyperphosphorylation Pathway and Alzheimer's Disease, Neurofibrillary Tangles, Neurodegenerative Disorders, Nerve Degeneration, Neoplasms. The study of the Hyperphosphorylation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Hyperphosphorylation Pathway has been researched in relation to Cell Cycle, Pathogenesis, Cell Death, Cell Proliferation, Localization. The Hyperphosphorylation Pathway complements our catalog of research reagents including antibodies and ELISA kits against AKT1, APP, CCND1, CDK1, CDK2.

Hyperphosphorylation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Hyperphosphorylation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1910 products for the study of the Hyperphosphorylation Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NBP2-25162
Western Blot: Tau Antibody (2E9) [NBP2-25162] - Blots of crude rat brain extract, revealing, as expected, multiple bands in the range 48-67kDa.Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF

NBP2-15843
Western Blot: Cdk5 Antibody [NBP2-15843] - Sample (50 ug of whole cell lysate)  A: mouse brain 12% SDS PAGE  diluted at 1:1000 Immunocytochemistry/Immunofluorescence: Cdk5 Antibody [NBP2-15843] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-13075
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid  (MOAB-2, Lanes 3 and 4).  Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice.  Scale bar 20 um (from PMID: 22423893).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

9 Publications
NB100-56598
Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IHC-Fr

2 Publications
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

11 Publications
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NBP2-37507
Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Western blot analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.Immunocytochemistry/Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Immunofluorescence analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, Flow

     1 Review

NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-AKT1 (NBP2-01725) and 2 ug/ml pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

8 Publications
NBP1-47470
Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

     3 Reviews

2 Publications
AF7250
CDK5 Activator 1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human CDK5 Activator 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7250) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent‑Basic (Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human
Applications IHC

NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

3 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     2 Reviews

22 Publications
NBP1-31308
Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody  diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
NBP2-24695
Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody  and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.

Rabbit Polyclonal
Species Human
Applications WB, IHC-P

2 Publications
NBP1-87232
Western Blot: PPP2R4 Antibody [NBP1-87232] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/Immunofluorescence: PPP2R4 Antibody [NBP1-87232] - Staining of human cell line U-251 MG shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

AF1555
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human
Applications WB

     1 Review

11 Publications
AF467
        EphB2  was detected in immersion fixed paraffin-embedded sections of human  esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen  Affinity-purified Polyclonal Antibody (Catalog # AF467) at  3 µg/mL for 1 hour at room temperature followed by  incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody  (Catalog # <a class=    COLO 205  human colorectal adenocarcinoma cell line was stained with Goat  Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog  # AF467, filled histogram) or isotype control antibody (Catalog #<br><a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     4 Reviews

22 Publications
NB100-240
Western Blot: TOR/mTOR Antibody [NB100-240] - Detection of Human mTOR by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa, and (50 ug) 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody NB100-240 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.   Affinity purified rabbit anti-mTOR antibody used for WB at 0.4 ug/ml (A) used for IP at 3 ug/mg lysate. mTOR was also immunoprecipitated by a previous lot of this antibody and by rabbit anti-mTOR antibodies NB100-241 and NB100-58821.  For blotting immunoprecipitated mTOR, NB100-58821 was used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).

Rabbit Polyclonal
Species Human, Rat
Applications WB, ICC/IF, IHC

5 Publications