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Focal Adhesion Assembly Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Focal Adhesion Assembly Pathway and Tissue Adhesions, Malignant Neoplasms, Neoplasms, Cell Invasion, Carcinoma. The study of the Focal Adhesion Assembly Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Focal Adhesion Assembly Pathway has been researched in relation to Cell Adhesion, Cell Migration, Cell Motility, Localization, Integrin Activation. The Focal Adhesion Assembly Pathway complements our catalog of research reagents including antibodies and ELISA kits against AKT1, C3, PTK2B, FN1, ILK.

Focal Adhesion Assembly Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Focal Adhesion Assembly below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1462 products for the study of the Focal Adhesion Assembly Pathway that can be applied to Chromatin Immunoprecipitation, Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

NSB624
Western Blot: FAK [p Tyr397] Antibody [NSB624] - Analysis of CEF cells illustrate that only the phosphopeptide corresponding to FAK (pY397) blocks the Western blot signal.Immunocytochemistry/Immunofluorescence: FAK [p Tyr397] Antibody [NSB624] - Visualizing focal adhesion kinase with FAK (pY397) PSSA. HeLa cells were fixed and permeabilized using ice-cold 95% methanol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

AF4259
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC

NB600-1293
Immunocytochemistry: Vinculin Antibody (hVIN-1) [NB600-1293] - Mouse primary keratinocytes stained with the clone hVIN-1. This image was submitted via customer Review.Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled at a 1:400 dilution.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

2 Publications
NBP1-91258
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Fibronectin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Bovine
Applications WB, Simple Western, ICC/IF

     5 Reviews

7 Publications
NBP2-07463
Western Blot: PXDN Overexpression Lysate (Adult Normal) [NBP2-07463] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PXDN.


Species Human

NBP1-47602
Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (1D4) [NBP1-47602] - analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.

Mouse Monoclonal
Species Bovine
Applications WB, ELISA, ICC/IF

1 Publication
NBP2-01462
Western Blot: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.Immunocytochemistry/Immunofluorescence: RhoD Antibody (2F7) [NBP2-01462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RhoD.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

3 Publications
NBP2-36561
Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]

Mouse Monoclonal
Species Human, Mouse
Applications WB, B/N, ELISA

7 Publications
NBP2-00532
Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.Immunocytochemistry/Immunofluorescence: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

AF808
Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=Recombinant Mouse Osteopontin/OPN (Catalog # <a class=

Goat Polyclonal
Species Mouse
Applications WB, IHC, ELISA(Cap)

     3 Reviews

44 Publications
NBP1-91228
Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ICC/IF, IHC

1 Publication
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

H00003611-M01
Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10. Analysis of ILK expression in NIH/3T3.Immunocytochemistry/Immunofluorescence: ILK Antibody (4F10) [H00003611-M01] - Analysis of monoclonal antibody to ILK on Jurkat cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

NB200-540
Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on  kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern.

Rat Monoclonal
Species Mouse
Applications WB, Flow, IA

     1 Review

1 Publication
NBP2-37477
Western Blot: Lipoprotein a Antibody (4H1) [NBP2-37477] - Western blot analysis using LPA mAb against human LPA recombinant protein. (Expected MW is 34.1 kDa)Immunocytochemistry/Immunofluorescence: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunofluorescence analysis of HepG2 cells using LPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-AKT1 (NBP2-01725) and 2 ug/ml pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

8 Publications
H00005706-M02
Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - PSMC6 monoclonal antibody (M02), clone 2C4. Analysis of PSMC6 expression in NIH/3T3.Immunocytochemistry/Immunofluorescence: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of monoclonal antibody to PSMC6 on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF