Submit your image related to Pathways to be featured!

Get Social

Submit your Twitter account related to Execution Phase Of Apoptosis to be featured!

Blogs

Submit your blog on Execution Phase Of Apoptosis to be featured!

Events

Submit your event on Execution Phase Of Apoptosis to be featured!

Videos

Submit your video on Execution Phase Of Apoptosis to be featured!

Charities

Submit your charity on Execution Phase Of Apoptosis to be featured!

Execution Phase Of Apoptosis Pathway Bioinformatics

Apoptosis is a form of programmed cell death, which can be either a developmental process or a response from the human immune system. During the execution phase of apoptosis, the cell undergoes changes including cell shrinkage, chromosomal condensation, nuclear and chromosomal DNA fragmentation, and blebbing. In particular, caspase-3 cleaves the DNAase inhibitor as well as proteins of the cytoskeleton, facilitating the process of cellular fragmentation. The execution phase occurs after the cells fully commit to die, and once this phase is initiated, cell death occurs very quickly. A preliminary step in this phase is microtubule disassembly through the depolymerization of actomyosin filaments, which helps to disperse cellular fragments and keep the plasma membrane, which results in the cellular packaging that keeps the apoptosis from becoming an inflammatory event. The final event of apoptosis is the phagocytosis of the apoptotic bodies by another cell, avoiding inflammation and any other adverse reactions to the cellular particles.

Execution Phase Of Apoptosis Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Execution Phase Of Apoptosis below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 2218 products for the study of the Execution Phase Of Apoptosis Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active).  Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST.  The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent.  Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, EM

     1 Review

153 Publications
NB100-56599
Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100.  The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution.  Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat (Negative)
Applications WB, Flow, ICC/IF

8 Publications
NB100-56503
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 ug of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using  NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

52 Publications
NB100-56098
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

     2 Reviews

10 Publications
NB100-56104
Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. Recombinant Bcl-XL (positive control).Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-Fr

     1 Review

1 Publication
NBP2-43728
Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [473]  diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: COL11A2 protein stained by COL11A2 antibody [473] diluted at 1:500. Blue: Hoechst 33342 staining.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF

NB100-56183
Western Blot: XIAP Antibody - Unpurified [NB100-56183] - Analysis of XIAP in various tumor cell lines using this antibody at 1:2000.Immunohistochemistry-Paraffin: XIAP Antibody - Unpurified [NB100-56183] - Human breast carcinoma using this antibody at 1:2000. A-A3, successively higher magnifications of the breast carcinoma tissue section. Hematoxylin-eosin counterstain.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

5 Publications
DFS00
 Fas/TNFRSF6/CD95 [HRP] Fas/TNFRSF6/CD95 [HRP]


Species Human

17 Publications
NBP1-28566
Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P <.05, ***P <.001 vs Sham group; #P <.05, ##P <.01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY licence.Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment.  IHC-F image submitted by a verified customer review

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, Func

     3 Reviews

12 Publications
NB100-56529
Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

1 Publication
NB100-56116
Immunohistochemistry: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Reduced pancreatic Atg7 level increased the expression of Caspase-8 in 12-week-old Atg7deltapan mice. Caspase-8 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-8 (green) ( 1/1000, scale bar=50 um). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY licence.Western Blot: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     1 Review

57 Publications
NB100-56074
Western Blot: APAF1 Antibody [NB100-56074] - Analysis of Apaf 1 using this antibody. Jurkat: human T-cell leukemia cell line lysate. RS11-846: human B-cell leukemia cell line lysate. Human thymus: tissue lysate.Immunocytochemistry/Immunofluorescence: APAF1 Antibody [NB100-56074] - Formalin-fixed, paraffin-embedded sections from a human brain tumor tissue microarray stained for Apaf 1 expression using NB100-56074 at 1:2000. A and B. Tissue microarray overview. A1 and B1, higher magnification of cores from A and B, respectively. Differential expression of Apaf 1 is seen between patient samples. Hematoxylin-eosin counterstain.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

28 Publications
NBP1-47602
Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.

Mouse Monoclonal
Species Human, Mouse, Bovine
Applications WB, ELISA, ICC/IF

3 Publications
AF860
Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IP

     4 Reviews

16 Publications