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Endothelial Cell Activation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Endothelial Cell Activation Pathway and Tissue Adhesions, Inflammation, Thrombosis, Atherosclerosis, Von Willebrand Disease. The study of the Endothelial Cell Activation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Endothelial Cell Activation Pathway has been researched in relation to Cell Activation, Cell Adhesion, Pathogenesis, Coagulation, Inflammatory Response. The Endothelial Cell Activation Pathway complements our catalog of research reagents including antibodies and ELISA kits against VCAM-1, CRP, CTLA4, F3, HLA-DQA1.

Endothelial Cell Activation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Endothelial Cell Activation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 2988 products for the study of the Endothelial Cell Activation Pathway that can be applied to Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

210-TA/CF
Recombinant Human TNF-alpha  (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human

428 Publications
575-ES


Species Mouse

28 Publications
796-IC


Species Mouse

48 Publications
NB600-586
Immunocytochemistry/Immunofluorescence: Von Willebrand Factor Antibody [NB600-586]Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

     2 Reviews

2 Publications
NBP2-15139
Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Tissue factor antibody diluted by 1:1000.Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Tissue Factor expression in paraffin-embedded human lung cancer tissues using Tissue Factor antibody. Image from verified customer review.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

     1 Review

NBP2-29323
Functional (Inhibition): NFkB p105/p50 Inhibitor Peptide Set [NBP2-29323] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29323) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).


Species Human, Mouse, Rat

10 Publications
NB100-1642
Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100.  The cells were incubated with anti-CD31/PECAM-1 (MEC 7.46) at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution.  Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution.  Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS-1 Cells with CD31 (MEC 7.46) antibody NB100-1642(blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).

Rat Monoclonal
Species Mouse
Applications Flow, ICC/IF, IHC

18 Publications
NBP2-42213
Western Blot: Thrombomodulin/BDCA-3 Antibody (6C8.2F9) [NBP2-42213] - Western blot analysis of Thrombomodulin/BDCA-3  [6C8.2F9] in mouse heart lysate at 5 ug/ml.Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (6C8.2F9) [NBP2-42213] - IHC analysis of a formalin fixed paraffin embedded tissue section of human placenta using Thrombomodulin/BDCA-3 antibody (clone 6C8.2F9) at 15 ug/ml concentration. The antibody generated specific staining in the endothelial cells of placental blood vessels while all other cell types including RBCs were found negative for Thrombomodulin.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, IHC

201-LB/CF
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED<SUB>50</SUB> for this effect is <12 pg/mL.1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.


Species Human

291 Publications
AF-386-PB
    Western  blot shows lysates of NS0 mouse myeloma cell line either mock transfected or  transfected with human <br>CTLA-4. PVDF membrane was probed with  0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen  Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

     2 Reviews

2 Publications
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

13 Publications
H00003117-M01
Proximity Ligation Assay: HLA DQA1 Antibody (1A3) [H00003117-M01] - Analysis of protein-protein interactions between CD74 and HLA-DQA1. HeLa cells were stained with anti-CD74 rabbit purified polyclonal 1:1200 and anti-HLA-DQA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).ELISA: HLA DQA1 Antibody (1A3) [H00003117-M01] - Detection limit for recombinant GST tagged HLA-DQA1 is 0.3 ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ELISA, PLA