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Cytoskeleton Organization Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Cytoskeleton Organization Pathway and Tissue Adhesions, Malignant Neoplasms, Neoplasms, Neoplasm Metastasis, Carcinoma. The study of the Cytoskeleton Organization Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Cytoskeleton Organization Pathway has been researched in relation to Actin Cytoskeleton Organization, Cell Adhesion, Cell Cycle, Cell Proliferation, Localization. The Cytoskeleton Organization Pathway complements our catalog of research reagents including antibodies and ELISA kits against AKT1, RHOA, CDC42, CDKN1A, EGF.

Cytoskeleton Organization Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Cytoskeleton Organization below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 2317 products for the study of the Cytoskeleton Organization Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP2-01462
Western Blot: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.Immunocytochemistry/Immunofluorescence: RhoD Antibody (2F7) [NBP2-01462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RhoD.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

NBP2-25159
Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159] - Pig retinal section stained with NBP2-25159 (green) and counterstained with rabbit polyclonal antibody to NF-M NB300-133 (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF

1 Publication
NB100-91273
Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

NBP1-91258
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Fibronectin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ICC/IF

     5 Reviews

5 Publications
NBP2-14907
Western Blot: CDC42 Antibody [NBP2-14907] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC42 antibody diluted at 1:500.Immunohistochemistry-Paraffin: CDC42 Antibody [NBP2-14907] - Paraffin-embedded mouse brain. CDC42 antibody  diluted at 1:500.

Rabbit Polyclonal
Species Mouse, Rat
Applications WB, IHC, IHC-P

     1 Review

NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-AKT1 (NBP2-01725) and 2 ug/ml pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

8 Publications
NBP2-45923
Western Blot: FAK Antibody (4D11) [NBP2-45923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAK.Immunohistochemistry: FAK Antibody (4D11) [NBP2-45923] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NB100-91266
Immunocytochemistry/Immunofluorescence: Rac1 Antibody [NB100-91266] -  Mouse Brain (cerebral cortex), Image is courtesy of customer review. Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat ligated sciatic nerve using Rabbit antibody to RAC1, RAC2 whole serum at 1:1000 dilution. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications ICC/IF, IHC, IHC-Fr

     1 Review

3 Publications
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NB100-2307
Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307] - Detection of Human Phospho WASP (S483/S484) by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho WASP (S483/S484) antibody NB100-2307 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307] - Whole cell  lysate (50 ug/lane) from U937 and Jurkat cells. Lysates were either untreated, mock treated with phosphatase or treated with phosphatase.  Antibody used at 1 ug/ml.

Rabbit Polyclonal
Species Human
Applications WB

6 Publications
NB600-1293
Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Mouse primary keratinocytes stained with the clone hVIN-1. Image is courtesy of customer review.Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled at a 1:400 dilution.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

2 Publications
AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

3 Publications
AF1095
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated<br>(‑) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=EGFR phosphorylated at Y1173 was detected in immersion fixed frozen sections of mouse embryo using Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

5 Publications
210-TA/CF
1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.Recombinant Human TNF-alpha  (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human

416 Publications
NBP2-00532
Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.Immunocytochemistry/Immunofluorescence: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

236-EG
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human

     7 Reviews

250 Publications
NB300-223
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - View of mixed neuron/glial cultures stained with NB300-223 (green) and  rabbit antibody to GFAP antibody NB300-141(red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.Western Blot: Vimentin Antibody [NB300-223] - Western blot of crude extract of human embryonic kidney Hek293 cells stained with NB300-223, showing a single strong clean band at approx. 50kDa.

Chicken Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

18 Publications
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

11 Publications
NB100-624
Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse Rho Kinase Beta (ROCK1/p160ROCK) on HeLa whole cell lysate using NB100-624. Rho Kinase Beta was also IPed using NB100-622 and NB100-623 at 3 ug/mg lysate.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     1 Review

3 Publications