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Cellular Response To Hypoxia Pathway Bioinformatics

Hypoxia occurs when a region of the body is deprived of oxygen. Hypoxia is a very dangerous condition when the brain is affected, for neurons can begin to die off within a matter of minutes, especially in cases of complete oxygen deprivation, or anoxia. Anaerobic metabolism occurs when the cells are not receiving oxygen, which results in a buildup of lactic acid, and could lead to cell death if oxygen is not soon introduced. Chemoreceptors in the carotid body are able to detect changes in the oxygen level of cells, and cause a response of vasodilation, or the widening of blood vessels to increase blood flow and allow for greater perfusion. Other methods of restoring oxygen levels include hyperventilation and adaptation, which is controlled by the hypoxia-inducible factor 1 (HIF-1). In response to hypoxia response elements, HIF-1 is able to control microRNAs, which can help the body adapt to low levels of oxygen through routes such as metabolic, apoptotic, proliferative, and angiogenic adaptations.

Cellular Response To Hypoxia Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Cellular Response To Hypoxia below! For more information on how to use Laverne, please read the How to Guide.
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Top Research Reagents

We have 2000 products for the study of the Cellular Response To Hypoxia Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout Hela cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ELISA

     32 Reviews

722 Publications
NBP2-65171


Species Human

NB100-122
Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Western blot of 786-O cells without or with VHL overexpression. Image from verified customer review.Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of HIF-2 alpha in MDA-MB-231 cell lysate (overexpression and endogenous samples) using anti-HIF-2 alpha antibody. The data showed that HIF-2 alpha antibody did not react to HIF-1 alpha overexpression. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     27 Reviews

528 Publications
NB100-124
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] -  Analysis of HIF-1 beta in HeLa nuclear extract.Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100.  The cells were incubated with anti-HIF-1 beta (H1beta234) at 5ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution.  Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution.  Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, GS

     1 Review

90 Publications
NB100-139
Western Blot: EGLN3/PHD3 Antibody [NB100-139] - Analysis of HeLa lysates using NB100-139. Image courtesy of Dr Gregg Semenza  (The Johns Hopkins University School of Medicine, Baltimore, MD USA)Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [NB100-139] - EGLN3/PHD3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, EM, ICC/IF

     2 Reviews

37 Publications
NB100-137
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of Human PHD2 by Western Blot. Samples : Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells. Antibody : Affinity purified rabbit anti-PHD2 used at 1 ug/ml. Detection : Chemiluminescence.Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of endogenous PHD2 in U2OS cells. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     9 Reviews

65 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     2 Reviews

22 Publications
AF231
    Western  blot shows lysates of HeLa human cervical epithelial carcinoma cell line and  MDA‑MB‑231 human breast cancer cell line. PVDF membrane  was probed with 1 µg/mL of Goat Anti-Human EGFR  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    A431  human epithelial carcinoma cell line was stained with Goat Anti-Human  EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog #  AF231, filled histogram) or isotype control antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Flow, IHC

     1 Review

7 Publications
NB100-485
Western Blot: Von Hippel Lindau Antibody [NB100-485] - 20 ug whole cell lysates (MCF7 human breast cancer cell line; K562 human leukemia cell line). Image from verified customer review.Western Blot: Von Hippel Lindau Antibody [NB100-485] - Detection of VHL in human kidney samples (30 ug)using NB 100-485. ECL detection 10 seconds. Lane 1: 0.5 ug/ml primary Lane 2: 2 ug/ml

Rabbit Polyclonal
Species Human
Applications WB, IP

     2 Reviews

5 Publications
NB600-607
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ELISA, IHC

5 Publications
NBP2-13967
Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NBP1-89150
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp.  Lane 4: Human plasma (IgG/HSA depleted).  Lane 5: Human liver tissue.  Lane 6: Human tonsil tissueImmunohistochemistry-Paraffin: ALDH9A1 Antibody [NBP1-89150] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

3 Publications
AF2574
    Human peripheral  blood monoctyes were stained with Mouse Anti-Human CD14  APC‑conjugated Monoclonal Antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications CyTOF-ready, ICFlow

NBP1-31302
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLD protein stained by DLD antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

AF6394
Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human EGLN2/PHD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6394) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human
Applications WB, IP

4 Publications
233-FB
1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.Recombinant Human FGF basic (146aa) (Catalog # 233-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED<sub>50</sub> for this effect is 0.1-0.6 ng/mL.


Species Human

     12 Reviews

249 Publications