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B Cell Activation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the B Cell Activation Pathway and Autoimmune Reaction, Autoimmune Diseases, Lupus Erythematosus, Systemic, Autoimmunity, Lymphoma. The study of the B Cell Activation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The B Cell Activation Pathway has been researched in relation to Cell Activation, Cell Proliferation, Secretion, B Cell Proliferation, Immune Response. The B Cell Activation Pathway complements our catalog of research reagents including antibodies and ELISA kits against AB, BCR, CD4, CD19, CD22.

B Cell Activation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on B Cell Activation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 3464 products for the study of the B Cell Activation Pathway that can be applied to Western Blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Immunohistochemistry from our catalog of antibodies and ELISA kits.

204-IL/CF
Recombinant Human IL-4 (Catalog # 204‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED<SUB>50</SUB> for this effect is 0.05‑02 ng/mL. 1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.


Species Human

259 Publications
202-IL/CF
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED<SUB>50</SUB> for this effect is 0.05-0.25 ng/mL.


Species Human

207 Publications
AF-386-PB
    Western  blot shows lysates of NS0 mouse myeloma cell line either mock transfected or  transfected with human <br>CTLA-4. PVDF membrane was probed with  0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen  Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

     2 Reviews

2 Publications
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

13 Publications
H00003117-M01
Proximity Ligation Assay: HLA DQA1 Antibody (1A3) [H00003117-M01] - Analysis of protein-protein interactions between CD74 and HLA-DQA1. HeLa cells were stained with anti-CD74 rabbit purified polyclonal 1:1200 and anti-HLA-DQA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).ELISA: HLA DQA1 Antibody (1A3) [H00003117-M01] - Detection limit for recombinant GST tagged HLA-DQA1 is 0.3 ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ELISA, PLA

DDX0130P-100
Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - Human cervical tissue. a: normal cervical epithelium. b: cervical intraepithelial neoplasia. c: squamous cell carcinoma.

Mouse Monoclonal
Species Human
Applications Flow, Func, ICC/IF

8 Publications
NBP1-19097
Western Blot: BCR Antibody [NBP1-19097] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Affinity purified rabbit anti-BCR antibody  used for IP at 10 mcg/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19098, which recognizes a downstream epitopeImmunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19097] -  Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene).  Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.

Rabbit Polyclonal
Species Human
Applications WB, Flow, ICC/IF

210-TA/CF
Recombinant Human TNF-alpha  (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human

428 Publications
285-IF/CF
1 μg/lane of Recombinant Human IFN-gamma  was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.Recombinant Human IFN-gamma  (Catalog # 285‑IF/CF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED<SUB>50</SUB> for this effect is 0.15‑0.75 ng/mL.


Species Human

219 Publications
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

     6 Reviews

13 Publications
NBP2-02905
Western Blot: B7-1/CD80 Antibody (2E5) [NBP2-02905] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80.

Mouse Monoclonal
Species Human, Monkey
Applications WB, ICC/IF, IHC

     1 Review

AF123
CD23/Fc epsilon  RII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD23/Fc epsilon  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <A class=

Goat Polyclonal
Species Human
Applications WB, Block, ICC

     1 Review

2 Publications
MAB4909
Western blot shows lysates of human spleen tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CD21 Monoclonal Antibody (Catalog # MAB4909) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, Flow, CyTOF-ready

1 Publication
NBP2-25196
Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

10 Publications
NBP2-46125
Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of extracts (35ug) from 9 different cell lines by using CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Mouse monoclonal
Species Human, Mouse, Rat
Applications WB, IF

     1 Review