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Autocrine Signaling Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Autocrine Signaling Pathway and Neoplasms, Malignant Neoplasms, Carcinoma, Cell Transformation, Neoplastic, Neoplasm Metastasis. The study of the Autocrine Signaling Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Autocrine Signaling Pathway has been researched in relation to Secretion, Cell Proliferation, Angiogenesis, Cell Growth, Paracrine Signaling. The Autocrine Signaling Pathway complements our catalog of research reagents including antibodies and ELISA kits against EGFR, VEGFA, IL6, EGF, TNF.

Autocrine Signaling Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Autocrine Signaling below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 3989 products for the study of the Autocrine Signaling Pathway that can be applied to Flow Cytometry, Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP2-50599
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

4 Publications
DVE00
 VEGF [HRP] VEGF [HRP]


Species Human

     25 Reviews

512 Publications
7270-IL/CF


Species Human

236-EG
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human

     19 Reviews

454 Publications
210-TA
1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human

     31 Reviews

645 Publications
294-HG
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED<sub>50</sub> for this effect is 0.1‑0.5 ng/mL.


Species Human

     4 Reviews

83 Publications
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog #<A class=NoLineLink href=Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

23 Publications
NBP2-22471
Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Human breast cancer cell MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected by western blot. From verified customer review.Immunocytochemistry/Immunofluorescence: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ICC/IF

     1 Review

3 Publications
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

MAB1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,<br>PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=    ERK2  was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast  adipose-like cell line using Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal  Antibody (Catalog # MAB1230) at 3 µg/mL for 3 hours at  room temperature. Cells were stained using the NorthernLights™  557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog #  <a class=

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

233-FB
Recombinant Human FGF basic/FGF2/bFGF (146 aa) (Catalog # 233-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The activity is approximately 3-fold greater than the top competitor's FGF basic (146 aa).1 µg/lane of Recombinant Human FGF basic/FGF2/bFGF (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human

     16 Reviews

367 Publications
DAR00
 Amphiregulin [HRP] Amphiregulin [HRP]


Species Human

     6 Reviews

8 Publications
AF527
    HGF R/c‑MET  was detected in immersion fixed HT‑29 human colon adenocarcinoma  cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right  panel) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified  Polyclonal Antibody (Catalog # AF527) at 5 µg/mL for 3  hours at room temperature. Cells were stained using the  NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody  (red; Catalog # <a class=HGF R/c‑MET was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Mouse
Applications WB, IHC, Block

     4 Reviews

21 Publications
1845-SH
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.


Species Human

     5 Reviews

36 Publications
AF644
Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (Catalog # <a class=VEGFR2 was detected in acetone fixed cryosections of mouse kidney tissue using Goat Anti-Mouse VEGFR2/KDR/Flk-1 Polyclonal Antibody (Catalog # AF644) for 50 minutes at room temperature. Tissues were stained with rabbit anti-goat secondary<br>antibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate Chromogen (red) followed by counterstaining with hematoxylin (blue). Experiments were carried out and the image was provided by Dr. Grietje Molema, University Medical Center Groningen, The Netherlands.

Goat Polyclonal
Species Mouse
Applications WB, Flow, IHC

     3 Reviews

48 Publications
NB110-68800
Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1).  Lane 1: K562 human Caucasian chronic myeloid leukemia cell line  Lane 2: RAJI human Burkitt lymphoma cell lineImmunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

Mouse Monoclonal
Species Human, Mouse
Applications WB, ICC/IF

AF467
    Simple  Western lane view shows lysates of COLO 205 human colorectal  adenocarcinoma cell line and MBA‑MB‑468 human breast  cancer cell line, loaded at 0.2 mg/mL. A specific band was detected  for EphB2 at approximately 139 and 146 kDa (as indicated) using  20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified  Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    Western  blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog #  <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     9 Reviews

33 Publications
MAB25031
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # <A class=NoLineLink href=CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

     11 Reviews

40 Publications
DVR100C
 VEGFR1/Flt-1 [HRP] VEGFR1/Flt-1 [HRP]


Species Human

     1 Review

65 Publications