Submit your image related to Pathways to be featured!

Get Social

Submit your Twitter account related to Anoikis to be featured!

Blogs

Submit your blog on Anoikis to be featured!

Events

Submit your event on Anoikis to be featured!

Videos

Submit your video on Anoikis to be featured!

Charities

Submit your charity on Anoikis to be featured!

Anoikis Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Anoikis Pathway and Malignant Neoplasms, Neoplasms, Tissue Adhesions, Neoplasm Metastasis, Carcinoma. The study of the Anoikis Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Anoikis Pathway has been researched in relation to Cell Death, Cell Adhesion, Cell Cycle, Cell Proliferation, Cell Growth. The Anoikis Pathway complements our catalog of research reagents including antibodies and ELISA kits against CASPASE 3, AKT1, BCL2, BCL2L1, CASP3.

Anoikis Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Anoikis below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 2043 products for the study of the Anoikis Pathway that can be applied to Chromatin Immunoprecipitation, Flow Cytometry, Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-AKT1 (NBP2-01725) and 2 ug/ml pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

8 Publications
NSB624
Western Blot: FAK [p Tyr397] Antibody [NSB624] - Analysis of CEF cells illustrate that only the phosphopeptide corresponding to FAK (pY397) blocks the Western blot signal.Immunocytochemistry/Immunofluorescence: FAK [p Tyr397] Antibody [NSB624] - Visualizing focal adhesion kinase with FAK (pY397) PSSA. HeLa cells were fixed and permeabilized using ice-cold 95% methanol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3.Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Western Blot Image of anti-Caspase 3 (31A1067).  Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST.  The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent.  Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, EM

     1 Review

113 Publications
NBP1-91258
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Fibronectin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Bovine
Applications WB, Simple Western, ICC/IF

     5 Reviews

7 Publications
AF231
    Western  blot shows lysates of HeLa human cervical epithelial carcinoma cell line and  MDA‑MB‑231 human breast cancer cell line. PVDF membrane  was probed with 1 µg/mL of Goat Anti-Human EGFR  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    A431  human epithelial carcinoma cell line was stained with Goat Anti-Human  EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog #  AF231, filled histogram) or isotype control antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Flow, IHC

6 Publications
NB100-56098
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with scrambled control siRNA (lane 1) or Bcl-2 siRNA (lane2). Image from verified customer review.Western Blot: Bcl-2 Antibody [NB100-56098] - WB analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).

Rabbit Polyclonal
Species Human, Mouse, Canine
Applications WB, IHC, IHC-P

     2 Reviews

6 Publications
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

NBP2-53088
Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

NB100-56116
Western Blot: active/cleaved Caspase 8 Antibody [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. Image from verified customer review.Immunohistochemistry: active/cleaved Caspase 8 Antibody [NB100-56116] -  Formalin-fixed, paraffin-embedded sections from a brain tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

34 Publications
AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

3 Publications
AF467
    COLO 205  human colorectal adenocarcinoma cell line was stained with Goat  Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog  # AF467, filled histogram) or isotype control antibody (Catalog #<br><a class=    D3  mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled  histogram) or isotype control antibody (Catalog #<br><a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     6 Reviews

23 Publications
NBP1-91228
Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ICC/IF, IHC

1 Publication
NB100-56104
Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.Immunohistochemistry-Paraffin: Bcl-Xl Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-X expression using this antibody at the bottom of the images were negative for Bcl-X expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-X expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-X expression.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-Fr

     1 Review

1 Publication
AF748
    Western  blot shows lysates of A431 human epithelial carcinoma cell line, A549 human  lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19  mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line.  PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse  E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody  (Catalog # <a class=    Simple  Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19  mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell  line, and MCF‑7 human breast cancer cell line, loaded at  0.2 mg/mL. A specific band was detected for E‑Cadherin at  approximately 128 kDa (as indicated) using 5 µg/mL of Goat  Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated  Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     4 Reviews

19 Publications
NBP2-46403
Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Mouse monoclonal
Species Human
Applications IHC

NBP1-33116
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - A. 30 ug PC-12 whole cell lysate/extract 5 % SDS-PAGE PI3K p110 beta antibody [N3C2], Internal dilution: 1:1000Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] -  Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
NBP2-15071
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3K gamma antibody [C3], C-term diluted at1:500.Immunohistochemistry-Paraffin: PI 3-Kinase p110 gamma Antibody [NBP2-15071] -  Paraffin-embedded Human Breast Cancer

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NBP2-46404
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Mouse Monoclonal
Species Human
Applications IHC, IHC-P