Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
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![Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Western-Blot-NB100-56508-img0003.jpg "Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml."){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-Cytometry-NB100-56508-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-Cytometry-NB100-56508-img0005.jpg){kind=icon}
![Flow (Cell Surface): RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis using the PE conjugate of NB100-56508. Staining of RANK in RAW cells using 0.5 ug of RANK antibody. Green histogram represents isotype control; red represents RANK antibody.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-(Cell-Surface)-NB100-56508-img0008.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-Cytometry-NB100-56508-img0009.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(64C1385.1)-Western-Blot-NBP2-24702-img0009.jpg "Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence."){kind=icon}
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Formalin-fixed paraffin-embedded tissue sections of human liver were probed for RANK/TNFRSF11A mRNA (ACD RNAScope Probe, catalog # 412281; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NBP2-24702) at 5 ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to plasma membrane.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(64C1385.1)-Dual-RNAscope-ISH-IHC-NBP2-24702-img0011.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from Raw 264.7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(64C1385.1)-Western-Blot-NBP2-24702-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis of antibody on (a) untreated BALB/c mouse splenocytes and (b) BALB/c mouse splenocytes stimulated with 5 ug/ml ConA for 72 hours. Cells were stained with 2 ug of antibody per 1x10^6 cells. Green represents isotype control; red represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(64C1385.1)-Flow-Cytometry-NBP2-24702-img0014.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Detection of RANK in RAW cells. Lane 1. detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or this antibody. RANK was detected by western blot using this antibody.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(64C1385.1)-Western-Blot-NBP2-24702-img0001.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis on RAW cells using 5 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(64C1385.1)-Flow-Cytometry-NBP2-24702-img0013.jpg){kind=icon}
![SDS-Page: Human sRank Receptor/TNFRSF11A Protein [NBP2-35272]](http://images.novusbio.com/fullsize2/Human-sRank-Receptor-TNFRSF11A-Protein-SDS-Page-NBP2-35272-img0001.jpg "SDS-Page: Human sRank Receptor/TNFRSF11A Protein [NBP2-35272]")
![Western Blot: RANK/TNFRSF11A Antibody [NB100-56396] - Analysis of RANK in RAW cell lysate.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-Western-Blot-NB100-56396-img0003.jpg "Western Blot: RANK/TNFRSF11A Antibody [NB100-56396] - Analysis of RANK in RAW cell lysate."){kind=icon}
![RANK/TNFRSF11A [Biotin]](DATA_RANK_DY683_ELISA_2179.jpg "RANK/TNFRSF11A [Biotin]"){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody [NBP1-85771] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-Western-Blot-NBP1-85771-img0006.jpg "Western Blot: RANK/TNFRSF11A Antibody [NBP1-85771] - Analysis in human cell line SCLC-21H."){kind=icon}
![Immunohistochemistry-Paraffin: RANK/TNFRSF11A Antibody [NBP1-85771] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-Immunohistochemistry-Paraffin-NBP1-85771-img0002.jpg){kind=icon}